FBX09 Overexpression Lysate

Images

 
Western Blot: FBX09 Overexpression Lysate (Adult Normal) [NBL1-10643] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FBX09.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

FBX09 Overexpression Lysate Summary

Description
Transient overexpression lysate of F-box protein 9 (FBXO9), transcript variant 3 The lysate was created in HEK293T cells, using Plasmid ID RC200561 and based on accession number NM_033481. The protein contains a C-MYC/DDK Tag.
Gene
FBXO9

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
47.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FBX09 Overexpression Lysate

  • Cross-immune reaction antigen 1
  • dJ341E18.2
  • F-box only protein 9
  • F-box protein 9
  • F-box protein Fbx9
  • FBX9DKFZp434C0118
  • KIAA0936
  • NY-REN-57
  • Renal carcinoma antigen NY-REN-57
  • VCIA1

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates at least 3 transcript variants diverging at the 5' terminus. Transcript Variant: This variant (3) contains a different segment for the 5' UTR and lacks part of the 5' coding region, compared to variant 1. It uses a downstream translation start codon, with the resulting protein (isoform 3) having a shorter N-terminus when it is compared to isoform 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol FBXO9