![Western Blot: Fbl14 Antibody [NBP2-33296] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/Fbl14-Antibody-Western-Blot-NBP2-33296-img0011.jpg "Western Blot: Fbl14 Antibody [NBP2-33296] - Analysis in human cell line HDLM-2.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit Fbl14 Antibody - BSA Free (NBP2-33296) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: TLNIGQCVRITDKGLELIAEHLSQLTGIDLYGCTRITKRGLERITQLPCLKVLNLGLWQMTDSEKEARGDFSPLFTVRTRGSS |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FBXL14 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Fbl14 Antibody [NBP2-33296] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/Fbl14-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33296-img0006.jpg "Immunocytochemistry/Immunofluorescence: Fbl14 Antibody [NBP2-33296] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.")
![Immunohistochemistry-Paraffin: Fbl14 Antibody [NBP2-33296] - Staining of human endometrium shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Fbl14-Antibody-Immunohistochemistry-Paraffin-NBP2-33296-img0014.jpg "Immunohistochemistry-Paraffin: Fbl14 Antibody [NBP2-33296] - Staining of human endometrium shows weak to moderate cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Fbl14 Antibody [NBP2-33296] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Fbl14-Antibody-Immunohistochemistry-Paraffin-NBP2-33296-img0007.jpg "Immunohistochemistry-Paraffin: Fbl14 Antibody [NBP2-33296] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.")
![Immunohistochemistry-Paraffin: Fbl14 Antibody [NBP2-33296] - Staining of human tonsil shows weak to moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Fbl14-Antibody-Immunohistochemistry-Paraffin-NBP2-33296-img0012.jpg "Immunohistochemistry-Paraffin: Fbl14 Antibody [NBP2-33296] - Staining of human tonsil shows weak to moderate cytoplasmic positivity in germinal center cells.")
![Immunohistochemistry-Paraffin: Fbl14 Antibody [NBP2-33296] - Staining of human skin shows weak to moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Fbl14-Antibody-Immunohistochemistry-Paraffin-NBP2-33296-img0013.jpg "Immunohistochemistry-Paraffin: Fbl14 Antibody [NBP2-33296] - Staining of human skin shows weak to moderate cytoplasmic positivity in squamous epithelial cells.")
![Wnt-5b [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Wnt-5b [Unconjugated]")
![Western Blot Snail Antibody [Unconjugated]](https://images.novusbio.com/images2/Snail_AF3639_Western_Blot_5320.jpg)
![Intracellular Staining by Flow Cytometry Snail Antibody [Unconjugated]](https://images.novusbio.com/images2/Snail_AF3639_Flow_Cytometry_20834.jpg)
![Chromatin Immunoprecipitation (ChIP) Snail Antibody [Unconjugated]](https://images.novusbio.com/images2/Snail_AF3639_Chromatin_Immunoprecipitation_11236.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")