Home » FATP5/SLC27A5 » FATP5/SLC27A5 Antibodies » FATP5/SLC27A5 Antibody (390917) [Alexa Fluor® 700]

FATP5/SLC27A5 Antibody (390917) [Alexa Fluor® 700]

 Submit a Review

Images

 
There are currently no images for FATP5/SLC27A5 Antibody (FAB3897N).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
390917
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB3897U-100UG
Alexa Fluor 405
FAB3897V-100UG
Alexa Fluor 488
FAB3897G-100UG
Alexa Fluor 594
FAB3897T-100UG
Alexa Fluor 647
FAB3897R-100UG
Alexa Fluor 750
FAB3897S-100UG
Unconjugated
MAB3897
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB3897
Carrier Free
MAB3897
Carrier Free
MAB3897-SP
Carrier Free
FAB3897V-100UG
Carrier Free
FAB3897U-100UG
Carrier Free
FAB3897T-100UG
Carrier Free
FAB3897S-100UG
Carrier Free
FAB3897R-100UG
Carrier Free
FAB3897G-100UG
100% Guarantee on every product

FATP5/SLC27A5 Antibody (390917) [Alexa Fluor® 700] Summary

Immunogen
HEK293 human embryonic kidney cell line transfected with human FATP5
Met1-Leu690
Accession # Q9Y2P5
Specificity
Detects human FATP5. Stains human FATP5 transfectants but not irrelevant transfectants.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SLC27A5
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FATP5/SLC27A5 Antibody (390917) [Alexa Fluor® 700]

  • ACSB
  • ACSBBACS
  • ACSVL6
  • ACSVL6BAL
  • BA-CoA ligase
  • Bile acid-CoA ligase
  • bile acyl-CoA synthetase
  • Cholate--CoA ligase
  • EC 6.2.1.7
  • FACVL3
  • FACVL3FATP-5
  • FATP5
  • FATP5Solute carrier family 27 member 5
  • Fatty acid transport protein 5
  • Fatty-acid-coenzyme A ligase, very long-chain 3
  • FLJ22987
  • SLC27A5
  • solute carrier family 27 (fatty acid transporter), member 5
  • VLACSR
  • VLACSRVery long-chain acyl-CoA synthetase homolog 2
  • VLCSH2
  • VLCSH2Very long-chain acyl-CoA synthetase-related protein
  • VLCS-H2VLACS-related

Background

FATP5, also known as BACS, VLACSR and SLC27A5, is a 75 kDa endoplasmic reticulum membrane protein that is expressed in hepatocytes. FATP5 is required for the conjugation of bile acids to CoA and for the uptake of long chain fatty acids into the liver. Human FATP5 shares 71% and 66% amino acid sequence identity with mouse and rat FATP5, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

FATP1/SLC27A1
Antibody (308420) [Un ...
MAB3304
FATP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human FATP1 Monoclonal Antibody (Catalog # MAB3304) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(2 Publications)
Species: Hu
Applications: CyTOF-ready, ICC, ICFlow
Dopamine D3R/DRD3
Antibody
NBP3-03577
Western Blot: Dopamine D3R/DRD3 Antibody [NBP3-03577] - Analysis of extracts of mouse brain, using Dopamine D3R/DRD3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunocytochemistry/Immunofluorescence: Dopamine D3R/DRD3 Antibody [NBP3-03577] - Analysis of U2OS cells using Dopamine D3R/DRD3 antibody. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Dopamine D3R/DRD3 Antibody [NBP3-03577] - Paraffin-embedded human breast cancer using Dopamine D3R/DRD3 antibody at dilution of 1:100 (40x lens).
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
FATP2/SLC27A2
Antibody (6B3A9)
NBP2-37738
Western Blot: FATP2/SLC27A2 Antibody (6B3A9) [NBP2-37738] - Analysis using SLC27A2 mAb against HEK293 (1) and SLC27A2 (AA: 346-405)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: FATP2/SLC27A2 Antibody (6B3A9) [NBP2-37738] - Analysis of esophageal cancer tissues using SLC27A2 mouse mAb with DAB staining.
Western Blot: FATP2/SLC27A2 Antibody (6B3A9) [NBP2-37738] - Analysis using SLC27A2 mAb against human SLC27A2 recombinant protein. (Expected MW is 32.4 kDa).
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
FATP4/SLC27A4
Antibody
NBP3-05510
Western Blot: FATP4/SLC27A4 Antibody [NBP3-05510] - Analysis in human cell line MCF-7.
Immunocytochemistry/Immunofluorescence: FATP4/SLC27A4 Antibody [NBP3-05510] - Staining of human cell line U-2 OS shows localization to vesicles.
Immunohistochemistry-Paraffin: FATP4/SLC27A4 Antibody [NBP3-05510] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
CCR5 Antibody
(45531) [Unconjugated
MAB182
Human peripheral blood mononuclear cells (PBMCs) were stained with either (A) Mouse Anti-Human CCR5 Monoclonal Antibody (Catalog # MAB182) or (B) Mouse IgG2B Isotype Control (Catalog # MAB0041) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.
Recombinant Human CCL3/MIP‑1 alpha (Catalog # 270-LD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL3/MIP‑1 alpha (40 ng/mL) is neutralized (green line) by increasing concentrations of Human CCR5 Monoclonal Antibody (Catalog # MAB182). The ND50 is typically 5-20 µg/mL.
CCR5 was detected in immersion fixed human dendritic cells using Human CCR5 Monoclonal Antibody (Catalog # MAB182) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
(1 Review)
(24 Publications)
Species: Hu
Applications: Flow, CyTOF-ready, ICC, Neut
FATP6/SLC27A6
Antibody
NBP2-81909
Western Blot: FATP6/SLC27A6 Antibody [NBP2-81909] - Analysis of FATP6/SLC27A6 in A20 cell lysate with FATP6/SLC27A6 antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/Immunofluorescence: FATP6/SLC27A6 Antibody [NBP2-81909] - Analysis of FATP6/SLC27A6 in rat heart tissue with FATP6/SLC27A6 antibody at 20 ug/ml.
Immunohistochemistry: FATP6/SLC27A6 Antibody [NBP2-81909] - Analysis of FATP6/SLC27A6 in rat heart tissue with FATP6/SLC27A6 antibody at 5 ug/ml.
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC
Adenosine
Deaminase/ADA Antibod
NBP1-87404
Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human duodenum, small intestine, stomach and testis using Anti-Adenosine Deaminase/ADA antibody NBP1-87404 (A) shows similar protein distribution across tissues to independent antibody NBP1-90361 (B).
Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Analysis in human duodenum and testis tissues using NBP1-87404 antibody. Corresponding Adenosine Deaminase/ADA RNA-seq data are presented for the same tissues.
Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
(4 Publications)
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, KD
BAAT Antibody
NBP2-15535
Western Blot: BAAT Antibody [NBP2-15535] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: BAAT Antibody [NBP2-15535] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.
Species: Hu
Applications: WB, IHC, IHC-P
CXCR4 Antibody
(44716) [Unconjugated
MAB172
Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
CXCR4 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Human CXCR4 Monoclonal Antibody (Catalog # MAB172) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172). The ND50 is typically 2.5-12 µg/mL.
(5 Reviews)
(45 Publications)
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Neut
Recombinant Human
POMT1 GST (N-Term) Pr ...
H00010585-Q01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
CD4 Antibody
NBP1-19371
Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.
Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.
(8 Reviews)
(29 Publications)
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
(33 Reviews)
(647 Publications)
Species: Hu
ABCB11 Antibody
NBP1-89319
Immunohistochemistry-Paraffin: ABCB11 Antibody [NBP1-89319] - Staining of human prostate shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: ABCB11 Antibody [NBP1-89319] - Staining of human liver shows moderate to strong membranous positivity in bile canaliculi.
Immunohistochemistry-Paraffin: ABCB11 Antibody [NBP1-89319] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected.
(5 Publications)
Species: Hu
Applications: IHC, IHC-P
HNF-4 alpha/NR2A1
Antibody
NBP1-89679
Western Blot: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Analysis in human cell lines Caco-2 and PC-3 using anti-HNF4A antibody. Corresponding HNF4A RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunocytochemistry/Immunofluorescence: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining of human cell line CACO-2 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Frozen: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Frozen 8 um rat liver tissue section fixed in formalin. HNF-4 alpha antibody at 1:100 (green). Multiplexed with a myeloperoxidase antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Insulin Antibody
(E2-E3 (same as INS04 ...
NBP2-34260
Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
(5 Reviews)
(1 Publication)
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
SLURP1 Antibody
AF4479
SLURP1 was detected in immersion fixed paraffin-embedded sections of human skin using Sheep Anti-Human SLURP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4479) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter­stained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Species: Hu
Applications: IHC
Desmoglein-3
Antibody (5H10)
NBP1-78984
Western Blot: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Colocalisation of Dsg3 with E-cadherin-catenin complex. Co-IPs demonstrated that both endogenous and exogenous Dsg3 interacted with the E-cadherin-catenin complex in A431 stable lines (the empty vector control (V) and hDsg3.myc (D3)) as well as in HaCaTs. When pulled down with antibodies against the myc tag or with a mouse Dsg3 Ab 5H10 and blotted for E-cadherin and beta-catenin, both proteins were detected in D3 and, to a lesser extent, in V. Conversely, when pulled down with E-cadherin Ab HECD-1 and blotted for Dsg3 or the myc tag and Pg, these proteins were detected in both V and D3 cells. Note that this result also was confirmed in HaCaTs (HaC) where Dsg3 was expressed endogenously (far right blot). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0014211), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984] - The cultured primary mouse skin keratinocytes were stained at 1:150. The image shows the cytosolic staining. This image was submitted via customer review.
Immunohistochemistry: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Analysis of Desmoglein 3 on human skin cancer using NBP1-78984.
(1 Review)
(7 Publications)
Species: Hu, Mu
Applications: WB, DB, ICC/IF, IHC, IHC-P, IP
Fatty Acid
Synthase/FASN Antibod
NB400-114
Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Expression of Fatty Acid Synthase in chicken hypothalamus. A: total RNA (1 ug) isolated from different tissues (brain, hypothalamus, liver and muscle) was subjected to RT-PCR using specific primers for chicken Fatty Acid Synthase (GenBank accession JO4485) or ribosomal 18S as a control (GenBank accession AF173612) B: tissue lysates (brain, hypothalmus, liver and muscle) were subjected to Western blot as described in MATERIALS AND METHODS. Blots were incubated with anti-Fatty Acid Synthase antibody and revealed by enhanced chemiluminescence. Picture compliments of Dridi S. et al, Am J Physiol Regul Integr Comp Physiol. 2006 Jul;291(1):R138-47. Epub 2006 Feb 2.
Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114] - The Fas antibody was tested in MCF-7 cells at a 1:2000 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NB400-114] - Immunohistochemical localization of FAS in chicken hypothalmus. Paraffin sections were obtained from 3-wk-old broiler chickens.
(2 Reviews)
(23 Publications)
Species: Hu, Mu, Rt, Po, Ch, Fe, Ha, Pl, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP, KD

Publications for FATP5/SLC27A5 Antibody (FAB3897N) (0)

There are no publications for FATP5/SLC27A5 Antibody (FAB3897N).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for FATP5/SLC27A5 Antibody (FAB3897N) (0)

There are no reviews for FATP5/SLC27A5 Antibody (FAB3897N). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FATP5/SLC27A5 Antibody (FAB3897N) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB3897U
Alexa Fluor 405 FAB3897V
Alexa Fluor 488 FAB3897G
Alexa Fluor 594 FAB3897T
Alexa Fluor 647 FAB3897R
Alexa Fluor 700 FAB3897N
Alexa Fluor 750 FAB3897S
Learn about Custom Antibody Labeling

Additional FATP5/SLC27A5 Products

Bioinformatics Tool for FATP5/SLC27A5 Antibody (FAB3897N)

Discover related pathways, diseases and genes to FATP5/SLC27A5 Antibody (FAB3897N). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FATP5/SLC27A5 Antibody (FAB3897N)

Discover more about diseases related to FATP5/SLC27A5 Antibody (FAB3897N).
 

Pathways for FATP5/SLC27A5 Antibody (FAB3897N)

View related products by pathway.

PTMs for FATP5/SLC27A5 Antibody (FAB3897N)

Learn more about PTMs related to FATP5/SLC27A5 Antibody (FAB3897N).

Blogs on FATP5/SLC27A5

There are no specific blogs for FATP5/SLC27A5, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Learn the difference between western blot and simple western

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FATP5/SLC27A5 Antibody (390917) [Alexa Fluor® 700] and receive a gift card or discount.


Bioinformatics

Gene Symbol SLC27A5
Uniprot