FAT4 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: FAT4 Antibody [NBP1-78381] - Antibody was tested in A431 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: FAT4 Antibody [NBP1-78381] - IHC analysis of FAT4 in mouse prostate cancer using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

FAT4 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human FAT4 protein (between residues 4000-4500) [UniProt Q6V0I7]
Localization
Membrane; Single-pass type I membrane protein. Note: In the kidney, localizes to primary cilia.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FAT4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
Application Notes
This FAT4 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Western blot reported in scientific literature (PMID: 29321622).
Publications
Read Publications using
NBP1-78381 in the following applications:

  • 3 publications
  • WB
    1 publication

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FAT4 Antibody

  • cadherin family member 14
  • cadherin-related family member 11
  • CDHR11
  • FAT tumor suppressor homolog 4 (Drosophila)
  • FAT-J
  • fat-like cadherin protein FAT-J
  • FLJ77878
  • hFat4
  • NBLA00548
  • putative protein product of Nbla00548

Background

FAT4 (FAT tumor suppressor homolog 4, also know as Fat-J, mFat4, and CDHF14) is a member of the Cadherin superfamily. Members of the Cadherin superfamily positively regulate planar cell polarity (PCP) in drosophila wings and play an important role in cell to cell adhesion interactions. FAT4 and other Cadherin family members have been used in systems medicine; especially in the fields of oncology and neurology (PMID: 16865240). FAT4 is widely expressed in most tissues, especially fetal and infant brain. It is also expressed in brain tumors and colorectal cancer. Furthermore, human breast tumor cell lines and primary tumors show a loss of FAT4 expression. Promoter methylation of a FAT4 allele can inactivate the expression of the FAT4 gene in deleted regions of the Hippo signaling pathway, the pathway that controls organ size (PMID: 19048595).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FAT4 Antibody (NBP1-78381)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(3)
WB
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
All Species

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FAT4 Antibody (NBP1-78381) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-78381AF350
Alexa Fluor 405 NBP1-78381AF405
Alexa Fluor 488 NBP1-78381AF488
Alexa Fluor 532 NBP1-78381AF532
Alexa Fluor 594 NBP1-78381AF594
Alexa Fluor 647 NBP1-78381AF647
Alexa Fluor 700 NBP1-78381AF700
Alexa Fluor 750 NBP1-78381AF750
Biotin NBP1-78381B
DyLight 350 NBP1-78381UV
DyLight 405 NBP1-78381V
DyLight 488 NBP1-78381G
DyLight 550 NBP1-78381R
DyLight 594 NBP1-78381DL594
DyLight 650 NBP1-78381C
DyLight 680 NBP1-78381FR
DyLight 755 NBP1-78381IR
FITC NBP1-78381F
Janelia Fluor 549 NBP1-78381JF549
Janelia Fluor 646 NBP1-78381JF646

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Bioinformatics Tool for FAT4 Antibody (NBP1-78381)

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Diseases for FAT4 Antibody (NBP1-78381)

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol FAT4
Entrez
Uniprot