FAT4 Antibody - BSA Free

Images

 
Genetic Strategies: Knockout Validated: FAT4 Antibody [NBP1-78381] - Effect of FAT4 knockout on GC tumorigenesis in the nude mice xenograft model. Representative images show IHC staining of BGC-823-KO-NC and ...read more
Genetic Strategies: Knockout Validated: FAT4 Antibody [NBP1-78381] - IHC staining of metastatic lung nodules in the nude mice model. Representative images show IHC staining with anti-FAT4 antibody of lung ...read more
Immunocytochemistry/ Immunofluorescence: FAT4 Antibody [NBP1-78381] - Antibody was tested in A431 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: FAT4 Antibody [NBP1-78381] - IHC analysis of FAT4 in mouse prostate cancer using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

FAT4 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human FAT4 protein (between residues 4000-4500) [UniProt Q6V0I7]
Localization
Membrane; Single-pass type I membrane protein. Note: In the kidney, localizes to primary cilia.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FAT4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Knockout Validated
  • Western Blot
Application Notes
This FAT4 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Western blot reported in scientific literature (PMID: 29321622).
Publications
Read Publications using
NBP1-78381 in the following applications:

  • 1 publication
  • 3 publications
  • WB
    1 publication

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FAT4 Antibody - BSA Free

  • cadherin family member 14
  • cadherin-related family member 11
  • CDHR11
  • FAT tumor suppressor homolog 4 (Drosophila)
  • FAT-J
  • fat-like cadherin protein FAT-J
  • FLJ77878
  • hFat4
  • NBLA00548
  • putative protein product of Nbla00548

Background

FAT4 (FAT tumor suppressor homolog 4, also know as Fat-J, mFat4, and CDHF14) is a member of the Cadherin superfamily. Members of the Cadherin superfamily positively regulate planar cell polarity (PCP) in drosophila wings and play an important role in cell to cell adhesion interactions. FAT4 and other Cadherin family members have been used in systems medicine; especially in the fields of oncology and neurology (PMID: 16865240). FAT4 is widely expressed in most tissues, especially fetal and infant brain. It is also expressed in brain tumors and colorectal cancer. Furthermore, human breast tumor cell lines and primary tumors show a loss of FAT4 expression. Promoter methylation of a FAT4 allele can inactivate the expression of the FAT4 gene in deleted regions of the Hippo signaling pathway, the pathway that controls organ size (PMID: 19048595).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FAT4 Antibody (NBP1-78381)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Flow, IHC-P, WB.


Filter By Application
Flow
(1)
IHC-P
(3)
WB
(1)
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Filter By Species
Human
(3)
Mouse
(2)
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Showing Publications 1 - 6 of 6.
Publications using NBP1-78381 Applications Species
Jiang X, Liu Z, Xia Y, Luo J. Low FAT4 expression is associated with a poor prognosis in gastric cancer patients. Oncotarget. 2018 Jan 12 [PMID: 29435168] (IHC-P, Mouse) IHC-P Mouse
Bliss SA, Paul S, Pobiarzyn PW et al. Evaluation of a developmental hierarchy for breast cancer cells to assess risk-based patient selection for targeted treatment Sci Rep 2018 Jan 10 [PMID: 29321622] (WB, Human) WB Human
Pujol F, Hodgson T, Martinez-Corral I et al. Dachsous1-Fat4 Signaling Controls Endothelial Cell Polarization During Lymphatic Valve Morphogenesis Arterioscler. Thromb. Vasc. Biol. 2017 Jul 13 [PMID: 28705793]
Jung HY, Cho H, Oh MH et al. Loss of FAT Atypical Cadherin 4 Expression Is Associated with High Pathologic T Stage in Radically Resected Gastric Cancer J Gastric Cancer 2015 Mar 01 [PMID: 25861521] (IHC-P, Human) IHC-P Human
Furukawa T, Sakamoto H, Takeuchi S et al. Whole exome sequencing reveals recurrent mutations in BRCA2 and FAT genes in acinar cell carcinomas of the pancreas Sci Rep 2015 Mar 06 [PMID: 25743105] (IHC-P, Human)

Details:
FAT4 antibody used for IHC-P analysis of paraffin processed tissue sections of human acinar cell carcinoma. The IHC-P assay was performed using streptavidin and biotin based detection kit namely - Histofine SAB-PO kit.
IHC-P Human
Hoelting TLB, Cidre-Aranaz F, Matzek D, Popper B Neomorphic DNA-binding enables tumor-specific therapeutic gene expression in fusion-addicted childhood sarcoma bioRxiv Jan 1 2022 (Flow, Mouse) Flow Mouse

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol FAT4
Entrez
Uniprot