FAT1 Antibody - BSA Free

Western Blot: FAT1 Antibody [NBP2-32275] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-FAT1 antibody NBP2-32275 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: WB, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

FAT1 Antibody - BSA Free Summary

• Immunogen: The immunogen this antibody was made to, maps to a region between residue 4484 to 4534 of human FAT Tumor Suppressor Homolog 1 using the numbering given in entry NP_005236.2 (GeneID 2195).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FAT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000 - 1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FAT1 Antibody - BSA Free

• FAT tumor suppressor 1
• FAT tumor suppressor homolog 1 (Drosophila)

Background

The Drosophila FAT 1 gene does not belong to the classical cadherin gene family yet encodes a transmembrane protein containing 34 cadherin repeats in association with a number of other motifs. The Drosophila FAT locus encodes a tumor suppressor gene and recessive (loss-of-function) mutations lead to hyperplastic overgrowth of the imaginal discs, indicating that contact dependent cell interactions may play an important role in regulating growth and cell adhesion. This excessive cell proliferation occurs while maintaining normal epithelial organization and differentiation potential. Expressed in many epithelial and some endothelial and smooth muscle cells. Humans contain many homologs of FAT including FAT1, or hFAT

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FAT1 Antibody (NBP2-32275)(1)

Publication using NBP2-32275

• Srivastava C, Irshad K, Gupta Y et al. NFkB is a critical transcriptional regulator of atypical cadherin FAT1 in glioma BMC Cancer 2020-01-28 [PMID: 31992226] (WB, Human)

Review for FAT1 Antibody (NBP2-32275) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 11/26/2018

Summary

• Application: Western Blot
• Sample Tested: H1944 lung adenocarcinoma cell line
• Species: Human
• Lot: a1

Bioinformatics

• Gene Symbol: FAT1
• Uniprot:
  • NP_005236.2(Human)