Human Fas/TNFRSF6/CD95 ELISA Kit (Colorimetric)

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ELISA: Fas/TNFRSF6/CD95 ELISA Kit [NBP1-91190] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum
Standard Curve Range
15.6 - 1,000 pg/mL
Sensitivity
13.2 pg/mL

Order Details

Human Fas/TNFRSF6/CD95 ELISA Kit (Colorimetric) Summary

Standard Curve Range
15.6 - 1,000 pg/mL
Sensitivity
13.2 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
3.10%
Intra-Assay
4.50%
Spike Recovery
100%
Sample Volume
10 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
FAS

Applications/Dilutions

Application Notes
The human sAPO-1/Fas ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sAPO-1/Fas.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Substrate Solution (tetramethyl-benzidine)
  2. Streptavidin-HRP
  3. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  4. Stop Solution (1M Phosphoric acid)
  5. Sample Diluent
  6. Human sAPO-1/Fas Standard lyophilized, 2 ng/ml upon reconstitution
  7. Biotin-Conjugate anti-human sAPO-1/Fas monoclonal antibody
  8. Adhesive Films
  9. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sAPO-1/Fas
  10. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)

Alternate Names for Human Fas/TNFRSF6/CD95 ELISA Kit (Colorimetric)

  • Apo-1 antigen
  • Apo-1
  • apoptosis antigen 1
  • Apoptosis-mediating surface antigen FAS
  • APT1
  • APT1FASTM
  • CD95 antigen
  • CD95
  • CD95ALPS1A
  • Fas (TNF receptor superfamily, member 6)
  • Fas AMA
  • Fas antigen
  • Fas
  • FAS1
  • FASLG receptor
  • TNFRSF6
  • TNFRSF6member 6
  • tumor necrosis factor receptor superfamily member 6

Background

APO-1/Fas (CD95), a member of the TNF/NGF receptor superfamily, is a glycosylated 48 kDa surface protein containing a single transmembrane region. APO-1 is expressed on a variety of human B and T cell lines, on many different tumor cells and on various normal human tissues. APO-1 also occurs in a soluble form (sAPO-1) devoid of a transmembrane region. Triggering of APO-1 by its ligand or by certain anti-APO- 1 monoclonal antibodies results in rapid induction of programmed cell death, apoptosis, in susceptible cells. The tissue distribution of APO-1 and of the APO-1 ligand suggests that the APO-1 receptor/ligand system plays an important role in various aspects of mammalian development and especially in the homeostasis of the immune system. Expression of the APO-1 cell surface protein is enhanced by IFN-gamma and TNF and by activation in lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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NBP1-91190
Species: Hu
Applications: ELISA

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Bioinformatics

Gene Symbol FAS
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