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Product Details

Reactivity HuSpecies Glossary
Applications ELISA
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Suitable Sample Type
Cell culture supernatant, serum

Order Details

Fas/TNFRSF6/CD95 ELISA Kit Summary

Standard Curve Range
15.6 - 1,000 pg/mL
13.2 pg/mL
Kit Type

Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.


Application Notes
The human sAPO-1/Fas ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sAPO-1/Fas.

Kit Components

  1. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sAPO-1/Fas
  2. Biotin-Conjugate anti-human sAPO-1/Fas monoclonal antibody
  3. Streptavidin-HRP
  4. Human sAPO-1/Fas Standard lyophilized, 2 ng/ml upon reconstitution
  5. Sample Diluent
  6. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  7. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  8. Substrate Solution (tetramethyl-benzidine)
  9. Stop Solution (1M Phosphoric acid)
  10. Adhesive Films

Reactivity Notes



Colored dyes for optional use with this kit are available and will be provided free of charge upon request. Please refer to catalog number NBP2-41404.

Alternate Names for Fas/TNFRSF6/CD95 ELISA Kit

  • Apo-1 antigen
  • Apo-1
  • apoptosis antigen 1
  • Apoptosis-mediating surface antigen FAS
  • APT1
  • CD95 antigen
  • CD95
  • CD95ALPS1A
  • Fas (TNF receptor superfamily, member 6)
  • Fas AMA
  • Fas antigen
  • FAS1
  • FASLG receptor
  • TNFRSF6member 6
  • tumor necrosis factor receptor superfamily member 6


APO-1/Fas (CD95), a member of the TNF/NGF receptor superfamily, is a glycosylated 48 kDa surface protein containing a single transmembrane region. APO-1 is expressed on a variety of human B and T cell lines, on many different tumor cells and on various normal human tissues. APO-1 also occurs in a soluble form (sAPO-1) devoid of a transmembrane region. Triggering of APO-1 by its ligand or by certain anti-APO- 1 monoclonal antibodies results in rapid induction of programmed cell death, apoptosis, in susceptible cells. The tissue distribution of APO-1 and of the APO-1 ligand suggests that the APO-1 receptor/ligand system plays an important role in various aspects of mammalian development and especially in the homeostasis of the immune system. Expression of the APO-1 cell surface protein is enhanced by IFN-gamma and TNF and by activation in lymphocytes.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Gene Symbol FAS

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