Fas/TNFRSF6/CD95 Antibody (431014) [Unconjugated]

Feline peripheral blood mononuclear cells were treated with PMA and Ca2+ ionomycin, then stained with Mouse Anti-Feline Fas/TNFRSF6/CD95 Mono­clonal Antibody (Catalog # MAB2267, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).

• Reactivity: Fe
• Applications: WB, Flow, CyTOF-ready
• Clone: 431014
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Fas/TNFRSF6/CD95 Antibody (431014) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant feline Fas/TNFRSF6/CD95
  Ala25-Lys172
  Accession # NP_001009314
• Specificity: Detects feline Fas/TNFRSF6/CD95 in direct ELISAs and Western blots. In direct ELISAs and Western blots no cross-reactivity with recombinant human Fas, recombinant mouse Fas, or recombinant rat Fas are observed
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FAS

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fas/TNFRSF6/CD95 Antibody (431014) [Unconjugated]

• Apo-1 antigen
• Apo-1
• apoptosis antigen 1
• Apoptosis-mediating surface antigen FAS
• APT1
• APT1FASTM
• CD95 antigen
• CD95
• CD95ALPS1A
• Fas (TNF receptor superfamily, member 6)
• Fas AMA
• Fas antigen
• Fas
• FAS1
• FASLG receptor
• TNFRSF6
• TNFRSF6member 6
• tumor necrosis factor receptor superfamily member 6

Background

Feline Fas (fibroblast associated; also named CD95 and APO-1) is a 45 kDa type I transmembrane (TM) glycoprotein that is a member of the TNF receptor superfamily (1-3). The family contains about 30 members, and is characterized by the presence of at least one cysteine-rich domain that contains multiple intrachain disulfide bonds. In general, the superfamily is divided into cytoplasmic death domain (DD) containing, and non-DD containing receptors (3). Feline Fas is synthesized as a 314 amino acid (aa) precursor that contains a 24 aa signal sequence, a 148 aa extracellular region, a 16 aa TM segment, and a 126 aa cytoplasmic tail (4). The extracellular region contains four potential N-linked glycosylation sites plus two distinct cysteine-rich domains of approximately 40 aa each; the cytoplasmic tail shows a 45 aa DD. The extracellular region of feline Fas shares 68%, 65%, 53%, and 58% aa sequence identity to porcine, human, mouse, and rat Fas, respectively. There are five alternate splice forms of feline Fas, which vary from 132 aa to 209 aa in length. All utilize exons 1-3 (aa 1-111) and all are missing the transmembrane segment of the full length form (5). Circulating Fas is reported to be both a dimer and trimer at low ng/mL concentrations. The ligand for Fas is FasL, and Fas ligation activates both the MEK cascade and FADD/caspase-8 pathway (7).

1. Locksley, R.M. et al. (2001) Cell 104:487.
2. Gaur, U. and B.B. Aggarwal (2003) Biochem. Pharmacol. 66:1403.
3. Collette, Y. et al. (2003) Trends Immunol. 24:387.
4. Mizuno, T. et al. (1998) Vet. Immunol. Immunopathol. 65:161.
5. Mizuno, T. et al. (2004) Eur. J. Immunogenet. 31:159.
6. Knipping, E. et al. (1995) Blood 85:1562.
7. Baker, S.J. and E.P. Reddy (1998) Oncogene 17:3261.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fas/TNFRSF6/CD95 Antibody (MAB2267)(1)

Publication using MAB2267

• WS Sprague, RM Troyer, X Zheng, BA Wood, M Macmillian, S Carver, S VandeWoude Prior Puma Lentivirus Infection Modifies Early Immune Responses and Attenuates Feline Immunodeficiency Virus Infection in Cats Viruses, 2018;10(4):. 2018 [PMID: 29677149] (Flow Cytometry, Feline)

Bioinformatics

• Gene Symbol: FAS
• Uniprot:
  • NP_001009314()