Fas/TNFRSF6/CD95 Antibody (431006) [Alexa Fluor® 647]

• Applications: WB
• Clone: 431006
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Fas/TNFRSF6/CD95 Antibody (431006) [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant feline Fas/TNFRSF6/CD95
  Ala25-Lys172
  Accession # NP_001009314
• Specificity: Detects feline Fas/TNFRSF6/CD95 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Fas, recombinant mouse Fas, or recombinant rat Fas is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Feline Fas (fibroblast associated; also named CD95 and APO-1) is a 45 kDa type I transmembrane (TM) glycoprotein that is a member of the TNF receptor superfamily, designated TNFRSF6 (1-3). The family contains about 30 members, and is characterized by the presence of at least one cysteine-rich domain that contains multiple intrachain disulfide bonds. In general, the superfamily is divided into cytoplasmic death domain (DD) containing, and non-DD containing receptors (3). Feline Fas is synthesized as a 314 amino acid (aa) precursor that contains a 24 aa signal sequence, a 148 aa extracellular region, a 16 aa TM segment, and a 126 aa cytoplasmic tail (4). The extracellular region contains four potential N-linked glycosylation sites plus two distinct cysteine-rich domains of approximately 40 aa each; the cytoplasmic tail shows a 45 aa DD. The extracellular region of feline Fas shares 68%, 65%, 53%, and 58% aa sequence identity to porcine, human, mouse, and rat Fas, respectively. There are five alternate splice forms of feline Fas, which vary from 132 aa to 209 aa in length. All use exons 1-3 (aa 1-111) and all are missing the transmembrane segment of the full length form (5). Circulating Fas is reported to be both a dimer and trimer at low ng/mL concentrations. The ligand for Fas is FasL, and Fas ligation activates both the MEK cascade and FADD/caspase-8 pathway (7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.