Fas Ligand/TNFSF6 Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC204894 and based on accession number NM_000639. The protein contains a C-terminal DDK tag.
Homo sapiens Fas ligand (TNF superfamily, member 6) (FASLG), mRNA.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Lysate Details for Fas Ligand/TNFSF6
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for Fas Ligand/TNFSF6 Overexpression Lysate (Native)
- apoptosis (APO-1) antigen ligand 1
- Apoptosis antigen ligand
- CD178 antigen
- Fas antigen ligand
- Fas ligand (TNF superfamily, member 6)
- Fas ligand
- FASLCD95 ligand
- tumor necrosis factor (ligand) superfamily, member 6
- tumor necrosis factor ligand superfamily member 6
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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FAQs for Fas Ligand/TNFSF6 Lysate (NBL1-10600). (Showing 1 - 1 of 1 FAQ).
I'd like to check toxicity of following products we recently ordered. 1)NBL1-10600 FASLG Overexpression Lysate, NBL1-11652 HNRNPU Overexpression Lysate 2)NB100-1935 CNPase Antibody. Do these products come with SDS buffer containing 2-mercaptoethanol?
- Both the FASLG Overexpression Lysate (NBL1-10600) and the HNRNPU Overexpression Lysate (NBL1-11652) contain: 1 vial of 100 ug gene specific transient over-expression cell lysate in RIPA buffer and 1 vial of 0.5 ml 2xSDS Sample Buffer (4%SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 5% b-mercaptoethanol).
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Bioinformatics Tool for Fas Ligand/TNFSF6 Lysate (NBL1-10600)
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