Fas Ligand/TNFSF6 Overexpression Lysate (Native)

Images

 
Western Blot: Fas Ligand Overexpression Lysate (Adult Normal) [NBL1-10600] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Fas Ligand.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Fas Ligand/TNFSF6 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204894 and based on accession number NM_000639. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Fas ligand (TNF superfamily, member 6) (FASLG), mRNA.
Gene
FASLG

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
31.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Fas Ligand/TNFSF6

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Fas Ligand/TNFSF6 Overexpression Lysate (Native)

  • apoptosis (APO-1) antigen ligand 1
  • Apoptosis antigen ligand
  • APT1LG1CD95L
  • APTL
  • CD178 antigen
  • CD178
  • CD95L
  • CD95-L
  • Fas antigen ligand
  • Fas ligand (TNF superfamily, member 6)
  • Fas Ligand
  • FASLCD95 ligand
  • FASLG
  • TNFSF6
  • TNFSF6FasL
  • tumor necrosis factor (ligand) superfamily, member 6
  • tumor necrosis factor ligand superfamily member 6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Fas Ligand/TNFSF6 Lysate (NBL1-10600) (0)

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FAQs for Fas Ligand/TNFSF6 Lysate (NBL1-10600). (Showing 1 - 1 of 1 FAQ).

  1. I'd like to check toxicity of following products we recently ordered. 1)NBL1-10600 FASLG Overexpression Lysate, NBL1-11652 HNRNPU Overexpression Lysate 2)NB100-1935 CNPase Antibody. Do these products come with SDS buffer containing 2-mercaptoethanol?
    • Both the FASLG Overexpression Lysate (NBL1-10600) and the HNRNPU Overexpression Lysate (NBL1-11652) contain: 1 vial of 100 ug gene specific transient over-expression cell lysate in RIPA buffer and 1 vial of 0.5 ml 2xSDS Sample Buffer (4%SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 5% b-mercaptoethanol).

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Bioinformatics

Gene Symbol FASLG
Entrez