Fas Ligand/TNFSF6 Antibody (252029) [Unconjugated]

Fas Ligand/TNFSF6 was detected in immersion fixed rat splenocytes using Mouse Anti-Rat Fas Ligand/TNFSF6 Monoclonal Antibody (Catalog # MAB1858) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Rt
• Applications: WB, ICC/IF
• Clone: 252029
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Fas Ligand/TNFSF6 Antibody (252029) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat Fas Ligand/TNFSF6
  Leu104-Leu278
  Accession # P36940
• Specificity: Detects rat Fas Ligand/TNFSF6 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse
  (rm) 4‑1BB Ligand, recombinant human (rh) APRIL, rmBAFF, rhEDA-A2, rmEDA, rhFas Ligand, rmFas Ligand, rhGITR Ligand, rmLIGHT, rhLymphotoxin alpha 1/ beta 2, rhLymphotoxin alpha 2/ beta 1, rmOX40Ligand, rhTL1, recombinant cotton rat TNF-alpha , rmTNF-alpha , rrTNF-alpha , rmTrail, rhTrance, and rmTWEAK is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FASLG
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fas Ligand/TNFSF6 Antibody (252029) [Unconjugated]

• apoptosis (APO-1) antigen ligand 1
• Apoptosis antigen ligand
• APT1LG1CD95L
• APTL
• CD178 antigen
• CD178
• CD95L
• CD95-L
• Fas antigen ligand
• Fas ligand (TNF superfamily, member 6)
• Fas Ligand
• FASLCD95 ligand
• FASLG
• TNFSF6
• TNFSF6FasL
• tumor necrosis factor (ligand) superfamily, member 6
• tumor necrosis factor ligand superfamily member 6

Background

Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a 40 kDa type II transmembrane member of the TNF superfamily of proteins. Its ability to induce apoptosis in target cells plays an important role in the development, homeostasis, and function of the immune system (1). Mature rat Fas Ligand consists of a 179 amino acid (aa) extracellular domain (ECD), a 22 aa transmembrane segment, and a 79 aa cytoplasmic domain. Within the ECD, rat Fas Ligand shares 78% and 93% aa sequence identity with human and mouse Fas Ligand, respectively. Fas Ligand is expressed as a nondisulfide-linked homotrimer on the surface of activated CD4⁺ Th1 cells, CD8⁺ cytotoxic T cells, and NK cells (1). Fas Ligand binding to Fas/CD95 on an adjacent cell triggers apoptosis in the Fas‑expressing cell (2, 4). Fas Ligand also binds DcR3 which is a soluble decoy receptor that interferes with Fas Ligand-induced apoptosis (5). Fas Ligand can be released from the cell surface by metalloproteinases as a 26 kDa soluble molecule which remains trimeric (6, 7). Shed Fas Ligand retains the ability to bind Fas, although its ability to trigger apoptosis is dramatically reduced (6, 7). In the absence of TGF‑ beta , however, Fas Ligand/Fas interactions instead promote neutrophil-mediated inflammatory responses (3, 8). Fas Ligand itself transmits reverse signals that costimulate the proliferation of freshly antigen-stimulated T cells (9). Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintance of immune privileged sites (10). This function is exploited by tumor cells which evade immune surveillance by upregulating Fas Ligand to kill tumor infiltrating lymphocytes (8, 11). In gld mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity (12, 13).

1. Lettau, M. et al. (2008) Curr. Med. Chem. 15:1684.
2. Takahashi, T. et al. (1994) Int. Immunol. 6:1567.
3. Seino, K-I. et al. (1998) J. Immunol. 161:4484.
4. Suda, T. et al. (1993) Cell 75:1169.
5. Pitti, R.M. et al. (1998) Nature 396:699.
6. Schneider, P. et al. (1998) J. Exp. Med. 187:1205.
7. Tanaka, M. et al. (1998) Nature Med. 4:31.
8. Chen, J.-J. et al. (1998) Science 282:1714.
9. Suzuki, I. and P.J. Fink (2000) Proc. Natl. Acad. Sci. 97:1707.
10. Ferguson, T.A. and T.S. Griffith (2006) Immunol. Rev. 213:228.
11. Ryan, A.E. et al. (2005) Cancer Res. 65:9817.
12. Takahashi, T. et al. (1994) Cell 76:969.
13. Lynch, D.H. et al. (1994) Immunity 1:131.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fas Ligand/TNFSF6 Antibody (MAB1858)(1)

Publication using MAB1858

• Jaime Palomino, Camila Gómez, María Teresa Otarola, Phillip Dettleff, Daniel Patiño-García, Renan Orellana et al. Embryo Buoyancy and Cell Death Gene Expression During Embryogenesis of Yellow-Tail Kingfish Seriola lalandi Frontiers in Cell and Developmental Biology 2021-03-18 [PMID: 33816479]

Bioinformatics

• Gene Symbol: FASLG
• Uniprot:
  • P36940()