FAK Antibody (10H7)

Images

 
Western Blot: FAK Antibody (10H7) [NBP2-22168] - Western blot analysis using FAK mouse mAb against A549 (1) and NIH/3T3 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: FAK Antibody (10H7) [NBP2-22168] - Immunofluorescence analysis of B16 cells using FAK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: FAK Antibody (10H7) [NBP2-22168] - Immunohistochemical analysis of paraffin-embedded cervices tumour using FAK mouse mAb with DAB staining

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
10H7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

FAK Antibody (10H7) Summary

Immunogen
Purified recombinant fragment of human FAK expressed in E. Coli.
Marker
Focal Adhesion Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PTK2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
119 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for FAK Antibody (10H7)

  • EC 2.7.10
  • FADK 1
  • FADK1
  • FADKFAK-related non-kinase polypeptide
  • FAK
  • FAK1FRNK
  • FAKEC 2.7.10.2
  • focal adhesion kinase 1
  • pp125FAK
  • Protein-tyrosine kinase 2
  • PTK2 protein tyrosine kinase 2
  • PTK2

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. Tissue specificity: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.RD: Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. It associates with several different signaling proteins such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and paxillin. This enables FAK to function within a network of integrin-stimulated signaling pathways leading to the activation of targets such as the ERK and JNK/mitogen-activated protein kinase pathways. FAK is also linked to oncogenes at biochemical and functional levels. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PTK2
Entrez