Factor XII Antibody

Immunohistochemistry-Paraffin: Factor XII Antibody [NBP1-52616] - Analysis of anti-F12 / Factor XII antibody with human liver at concentration 5 ug/ml.

Product Discontinued

Factor XII Antibody Summary

• Immunogen: Full length native protein (purified) (Human).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: F12
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:100 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -70C. Avoid freeze-thaw cycles.
• Buffer: 50mM Sodium Phosphate, 0.1M NaCl and 1mM EDTA, pH 6.6
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Factor XII Antibody

• beta-factor XIIa part 1
• coagulation factor XII (Hageman factor)
• coagulation factor XII
• coagulation factor XIIa heavy chain
• coagulation factor XIIa light chain
• EC 3.4.21
• EC 3.4.21.38
• F12
• HAE3
• HAEX
• HAF beta-factor XIIa part 2
• HAF
• Hageman factor

Background

Coagulation factor XII circulates in blood as a zymogen. This single chain zymogen is converted to a two chain serine protease with an heavy chain (alpha factor XIIa) and a light chain. The heavy chain contains two fibronectin type domains, two epidermal growth factor (EGF) like domains, a kringle domain and a proline rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta factor XIIa light chain and the alpha factor XIIa light chain becomes beta factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha factor XIIa and then to beta factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in Factor XII gene do not cause any clinical symptoms and the sole effect is that whole blood clotting time is prolonged.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: F12
• Entrez:
  • 2161(Human)
• Uniprot:
  • P00748(Human)