Factor XII Antibody

Product Discontinued

Factor XII Antibody Summary

• Immunogen: Human Factor XII purified from citrated human plasma. Greater than 95% purity by SDS-PAGE.
• Localization: Secreted
• Specificity: This product gives a single arc when tested by IEP against fresh human plasma.Identity has been confirmed by double diffusion (Ouchterlony) against human Factor XII and a known anti-human Factor XII.
• Clonality: Polyclonal
• Host: Sheep
• Gene: F12
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunofluorescence
  • Radioimmunodiffusion
• Application Notes: Double diffusion: 5uL antiserum vs 50uL fresh plasmaIEP: 100uL antiserum (diluted

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Glycine ed saline (pH 7.4), 0.01% Benzamidine, 0.1% EACA and 1.0mM EDTA
• Preservative: 0.099% Sodium Azide
• Purity: IgG purified

Alternate Names for Factor XII Antibody

• beta-factor XIIa part 1
• coagulation factor XII (Hageman factor)
• coagulation factor XII
• coagulation factor XIIa heavy chain
• coagulation factor XIIa light chain
• EC 3.4.21
• EC 3.4.21.38
• F12
• HAE3
• HAEX
• HAF beta-factor XIIa part 2
• HAF
• Hageman factor

Background

Coagulation factor XII circulates in blood as a zymogen. This single chain zymogen is converted to a two chain serine protease with an heavy chain (alpha factor XIIa) and a light chain. The heavy chain contains two fibronectin type domains, two epidermal growth factor (EGF) like domains, a kringle domain and a proline rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta factor XIIa light chain and the alpha factor XIIa light chain becomes beta factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha factor XIIa and then to beta factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in Factor XII gene do not cause any clinical symptoms and the sole effect is that whole blood clotting time is prolonged.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: F12
• Entrez:
  • 2161(Human)