Human Factor V ELISA Kit (Colorimetric)

ELISA: Human Factor V ELISA Kit (Colorimetric) [NBP2-82505] - Samples were spiked with high concentrations of Human Factor V and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Factor V ELISA Kit (Colorimetric) [NBP2-82505] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 62.5 - 4000 pg/mL
• Sensitivity: 37.5 pg/mL

Human Factor V ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Factor V in samples. No Significant cross-reactivity or interference between Human Factor V and analogues was observed.
• Standard Curve Range: 62.5 - 4000 pg/mL
• Sensitivity: 37.5 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.19
• Intra-Assay: CV% < 5.43
• Spike Recovery: 87-106%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: F5

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Factor V ELISA Kit (Colorimetric)

• Activated protein C cofactor
• Cf5
• Cf-5
• Coagulation Factor 5
• coagulation factor V (proaccelerin, labile factor)
• coagulation factor V jinjiang A2 domain
• Coagulation Factor V
• F5
• factor V Leiden
• Factor V
• FVL
• PCCF
• Proaccelerin, Labile Factor
• RPRGL1
• THPH2

Background

The F5 gene encodes a 2,224 amino acid long, 251 kDA coagulation factor V protein that is critical in the regulation of homeostasis. Additionally, F5 functions as a cofactor for the prothrombinase activity of factor Xa which results in the initiation of prothrombin to thrombin. F5 participates in blood coagulation signaling pathways, the blood clotting cascade, platelet degranulation as well as activation, signaling, and aggregation, and in responses to elevated platelet cytosolic Ca2+. It interacts with genes PROC, PROS1, F2, MMRN1, and CALR. Defects in F5 cause factor 5 deficiency (owren parahemophilia), thrombophilia due to activated protein C resistance, susceptibility to Budd-Chiari syndrome, susceptibility to ischemic strokes, and susceptibility to pregnancy loss, recurrent, type 1. F5 is also linked to deep vein thrombosis, antithrombin III deficiency, retinal vein occlusion, legg-calve-perthes disease, liver disease, hypertension, pulmonary embolism, lupus, and patent foramen ovale.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Factor V ELISA Kit (NBP2-82505). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: F5