MAB1050, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (
F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (
FC005). Staining was performed using our Staining Intracellular Molecules protocol." class="big_lightbox">

MAB1050, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (
F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (
FC005). Staining was performed using our Staining Intracellular Molecules protocol." alt="HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Coagulation Factor V Monoclonal Antibody (Catalog # MAB9856, filled histogram) or isotype control antibody (
MAB1050, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (
F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (
FC005). Staining was performed using our Staining Intracellular Molecules protocol." class="big_thumb" />