FACA/FANCA Antibody [DyLight 405]

Product Discontinued

FACA/FANCA Antibody [DyLight 405] Summary

• Immunogen: Synthetic peptide made to an internal portion of human FANCA (within residues 800-900). [UniProt# O15360]
• Localization: Major- Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FANCA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This FANCA antibody is useful for Western Blot, where a band can be seen at ~162 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for FACA/FANCA Antibody [DyLight 405]

• FA
• FA1
• FAA
• FAAMGC75158
• FACA
• FACAFanconi anemia group A protein
• FAH
• FA-H
• FANCA
• FANCH
• Fanconi anemia, complementation group A
• Fanconi anemia, complementation group H
• Fanconi anemia, type 1
• Protein FACA

Background

FANCA (Fanconi anemia group A protein) is a DNA repair protein which plays a key role in post-replication repair, cell cycle checkpoint function, interstrand DNA cross-link repair and maintenance of chromosome stability. FANCA belongs to multisubunit FA complex (FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM) which is absent in FA patients. When complexed with FANCF, FANCG and FANCL (but not with FANCC, nor FANCE), FANCA interacts with HES1 and this interaction is crucial for the stability as well as nuclear localization of FA core complex proteins. FANCA-FANCC/FANCG complex may include EIF2AK2 and HSP70 also and it directly interacts with FAAP20/C1orf86. Localized mainly in the nucleus and cytoplasm, it gets phosphorylated upon DNA damage through ATM/ATR. FANCA is a most frequently mutated gene in FA (representing 60-70% of the cases) and over 350 unique mutations have been reported which includes large genomic deletions mediated by unusually high density of ALU repetitions found in its genomic sequence. FANCA sequence alterations or its altered expression has been associated with ovarian cancer and leukemia, and homozygous mutations of FANCA have been linked to esophageal cancer. Defects in FANCA are a cause of Fanconi anemia (FA), a rare disorder characterized by physical abnormalities, bone marrow failure (BMF), increased risk of malignancies, and cellular hypersensitivity to DNA cross-linking agents.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FANCA