FABP3/H-FABP Overexpression Lysate (Native)

Images

 
Western Blot: cardiac FABP Overexpression Lysate (Adult Normal) [NBL1-10419] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for cardiac FABP.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

FABP3/H-FABP Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202737 and based on accession number NM_004102. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor) (FABP3), mRNA.
Gene
FABP3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
14.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for FABP3/H-FABP

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FABP3/H-FABP Overexpression Lysate (Native)

  • FABP11
  • FABP3
  • fatty acid binding protein 11
  • fatty acid binding protein 3, muscle and heart (mammary-derived growthinhibitor)
  • Fatty acid-binding protein 3
  • Fatty acid-binding protein 3, muscle
  • fatty acid-binding protein, heart
  • Heart-type fatty acid-binding protein
  • HFABP
  • H-FABP
  • H-FABPM-FABP
  • Mammary-derived growth inhibitor
  • MDGI
  • Muscle fatty acid-binding protein
  • O-FABP

Background

The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for FABP3/H-FABP Lysate (NBL1-10419) (0)

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Bioinformatics

Gene Symbol FABP3
Entrez