FABP3/H-FABP Antibody (67D3)

Product Discontinued

FABP3/H-FABP Antibody (67D3) Summary

• Immunogen: This FABP3/H-FABP Antibody (67D3) was developed against purified human H-FABP
• Specificity: This monoclonal antibody (subclass IgG1) binds to human heart Fatty Acid Bindings Protein. It shows no binding to human intestinal or liver FABP. The antibody stains heart muscle cells and striated skeletal muscle cells in immunohistochemistry. The antibody can be used to detect ischemia areas of human heart. The antibody is cross reactive with rat and mouse H-FABP.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FABP3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:50-1:100
• Application Notes: This antibody can be used for immuno assays, Western blotting, immunohistochemistry on frozen and paraffin embedded sections and immuno precipitation.For immunohistochemistry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for FABP3/H-FABP Antibody (67D3)

• FABP11
• FABP3
• fatty acid binding protein 11
• fatty acid binding protein 3, muscle and heart (mammary-derived growthinhibitor)
• Fatty acid-binding protein 3
• Fatty acid-binding protein 3, muscle
• fatty acid-binding protein, heart
• Heart-type fatty acid-binding protein
• HFABP
• H-FABP
• H-FABPM-FABP
• Mammary-derived growth inhibitor
• MDGI
• Muscle fatty acid-binding protein
• O-FABP

Background

The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FABP3