Ezrin Antibody

Western Blot: Ezrin Antibody [NB100-40810] - Staining of Human Placenta lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Ezrin Antibody [NB100-40810] - Staining of paraffin embedded Human Small Intestine. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: Ezrin Antibody [NB100-40810] - Staining of paraffin embedded Human Kidney. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Discontinued

Ezrin Antibody Summary

• Immunogen: Peptide with sequence C-QDYEEKTKKAER corresponding to internal region according to NP_003370.2.
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EZR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/mL
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/mL
• Application Notes: WB: Approx 80 kDa band observed in human placenta lysates (calculated MW of 69.4 kDa according to NP_003370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (PMID: 15788482). IHC: Paraffin embedded colon shows strong staining of enterocytes. Paraffin embedded Human Kidney.
• Control:

  Ezrin Overexpression Lysate
  Human Placenta Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ezrin Antibody

• CVIL
• CVL
• Cytovillin 2
• Cytovillin
• DKFZp762H157
• EZR
• Ezrin
• FLJ26216
• p81
• VIL2
• VIL2MGC1584
• villin 2 (ezrin)
• Villin 2
• villin-2

Background

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. It plays a key role in cell surface structure adhesion, migration, and organization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ezrin Antibody (NB100-40810)(2)

Publications using NB100-40810

• Stanasila L, Abuin L, Diviani D, Cotecchia S. Ezrin directly interacts with the alpha1b-adrenergic receptor and plays a role in receptor recycling. J Biol Chem 2006-02-17 [PMID: 16352594]
• Zhu L, Liu Y, Forte JG et al. Ezrin oligomers are the membrane-bound dormant form in gastric parietal cells. Am J Physiol Cell Physiol 2005-06-01 [PMID: 15788482]

Bioinformatics

• Gene Symbol: EZR
• Entrez:
  • 7430(Human)
  • 22350(Mouse)
  • 54319(Rat)
• Uniprot:
  • NP_003370.2(Human)