Ezrin Antibody (JM10-65)

Western Blot: Ezrin Antibody (JM10-65) [NBP2-66827] - Analysis of Ezrin on different cell lysates using anti-Ezrin antibody at 1/1,000 dilution. Positive control: Lane 1:Hela Lane 2: Jurkat Lane 3:A431

Immunohistochemistry-Paraffin: Ezrin Antibody (JM10-65) [NBP2-66827] - Analysis of paraffin-embedded human lung tissue using anti-Ezrin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Ezrin Antibody (JM10-65) [NBP2-66827] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Ezrin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Ezrin Antibody (JM10-65) [NBP2-66827] - Analysis of paraffin-embedded human colon cancer tissue using anti-Ezrin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Ezrin Antibody (JM10-65) [NBP2-66827] - Analysis of paraffin-embedded human kidney tissue using anti-Ezrin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Ezrin Antibody (JM10-65) [NBP2-66827] - Analysis of paraffin-embedded human liver cancer tissue using anti-Ezrin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Ezrin Antibody (JM10-65) [NBP2-66827] - Analysis of paraffin-embedded human lung cancer tissue using anti-Ezrin antibody. Counter stained with hematoxylin.

Product Discontinued

Ezrin Antibody (JM10-65) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Ezrin aa 350-386 / 586. (SwissProt: P15311 Human)
• Localization: Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EZR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Ezrin Antibody (JM10-65)

• CVIL
• CVL
• Cytovillin 2
• Cytovillin
• DKFZp762H157
• EZR
• Ezrin
• FLJ26216
• p81
• VIL2
• VIL2MGC1584
• villin 2 (ezrin)
• Villin 2
• villin-2

Background

Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. It is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity and is expressed in epithelial cells but not in mesenchymal cells. It is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EZR