EZH2/KMT6 Antibody

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Western Blot: KMT6/EZH2 Antibody [NB110-83976] - Detection of E2H2 in Jurkat whole cell lysate using NB110-83976.

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Catalog# & Conjugate Size Price

EZH2/KMT6 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of the human KMT6/EZH2 protein (within residues 300-400). [Swiss-Prot# Q15910]
Localization
Nuclear.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EZH2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
Application Notes
This KMT6/EZH2 antibody is useful for Western blot, where a band is seen ~ 85 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
EZH2/KMT6 Overexpression Lysate
Jurkat Whole Cell Lysate
EZH2/KMT6 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB110-83976 in the following application:

Reactivity Notes

Human, mouse and primate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EZH2/KMT6 Antibody

  • EC 2.1.1
  • EC 2.1.1.43
  • enhancer of zeste (Drosophila) homolog 2
  • enhancer of zeste 2
  • enhancer of zeste homolog 2 (Drosophila)
  • Enhancer of zeste homolog 2
  • ENX1
  • ENX-1KMT6EZH1KMT6A
  • EZH2
  • histone-lysine N-methyltransferase EZH2
  • KMT6
  • Lysine N-methyltransferase 6
  • MGC9169

Background

EZH2 (histone methyltransferase enhancer of zeste homolog 2) is a member of polycomb group proteins, and together with embryonic ectoderm development protein and suppressor of Zeste (SUZ12), EZH2 forms the polycomb repressor complex 2 which plays an important role in variety of biological processes such as differentiation, maintainance of cellular identity and proliferation, and stem-cell plasticity. EZH2 contains histone methyltransferase activity and mediates transcriptional repression of target genes at chromatin level by catalyzing trimethylation of histone H3 lysine 27 (H3K27me3). EZH2 is overexpressed in cancers of diverse origins that includes hematologic malignancies, urothelial/esophageal carcinoma, glioma, renal cell carcinoma, NSCLC, and colorectal, breast and hepatic cancer. Moreover, high expression of EZH2 has been linked to aggressive tumor formation/poor prognosis and EZH2 contributes to the metastasis through the downregulation of E-cadherin by mediating histone H3 methylation. Accordingly, inhibition of EZH2 expression results in significantly decreased cell proliferation and increased G2-M arrest.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-83976
Species: Hu, Mu, Pm
Applications: WB

Publications for EZH2/KMT6 Antibody (NB110-83976) (0)

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Review for EZH2/KMT6 Antibody (NB110-83976) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-83976:
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Images Ratings Applications Species Date Details
Western Blot EZH2/KMT6 NB110-83976
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reviewed by:
Anonymous
WB Human 07/25/2014
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Summary

ApplicationWestern Blot
Sample TestedHeLa cell lysates
SpeciesHuman

Blocking

Blocking Details5% milk

Primary Anitbody

Dilution Ratio1:500

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG-HRP
Secondary Concentration1:5000

Details

Detection NotesECL prime

Product General Protocols

View specific protocols for EZH2/KMT6 Antibody (NB110-83976): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EZH2/KMT6 Antibody (NB110-83976) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 NB110-83976V

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Bioinformatics Tool for EZH2/KMT6 Antibody (NB110-83976)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
07/25/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol EZH2
Entrez
Uniprot