EZH2/KMT6 Antibody (144CT2.1.1.5)

Images

 
Western Blot: EZH2/KMT6 Antibody (144CT2.1.1.5) [NBP2-29965] - All lanes : Anti-EZH2 Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: T47D whole cell lysate ...read more
Immunocytochemistry/ Immunofluorescence: EZH2/KMT6 Antibody (144CT2.1.1.5) [NBP2-29965] - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial ...read more
Immunohistochemistry: EZH2/KMT6 Antibody (144CT2.1.1.5) [NBP2-29965] - EZH2/KMT6 Antibody staining EZH2 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded ...read more
Immunocytochemistry/ Immunofluorescence: KMT6/EZH2 Antibody (144CT2.1.1.5) [NBP2-29965] - Fluorescent image of U251 cell stained with EZH2 Antibody(NBP2-29965).U251 cells were fixed with 4% PFA (20 min), permeabilized ...read more
Immunohistochemistry-Paraffin: KMT6/EZH2 Antibody (144CT2.1.1.5) [NBP2-29965] - immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the ...read more
Immunohistochemistry: EZH2/KMT6 Antibody (144CT2.1.1.5) [NBP2-29965] - EZH2/KMT6 Antibody staining EZH2 in human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
144CT2.1.1.5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.73 mg/ml

Order Details

EZH2/KMT6 Antibody (144CT2.1.1.5) Summary

Immunogen
This EZH2 antibody is generated from mouse immunized with EZH2 recombinant protein.
Localization
Cellular Location Nucleus. Tissue Location Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EZH2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:500
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:50
Theoretical MW
85.363 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-29965 in the following applications:

Reactivity Notes

Predicted to react with Monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
0.73 mg/ml
Purity
Protein G purified

Alternate Names for EZH2/KMT6 Antibody (144CT2.1.1.5)

  • EC 2.1.1
  • EC 2.1.1.43
  • enhancer of zeste (Drosophila) homolog 2
  • enhancer of zeste 2
  • enhancer of zeste homolog 2 (Drosophila)
  • Enhancer of zeste homolog 2
  • ENX1
  • ENX-1KMT6EZH1KMT6A
  • EZH2
  • histone-lysine N-methyltransferase EZH2
  • KMT6
  • Lysine N-methyltransferase 6
  • MGC9169

Background

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EZH2/KMT6 Antibody (NBP2-29965) (0)

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Review for EZH2/KMT6 Antibody (NBP2-29965) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-29965:
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Western Blot EZH2/KMT6 NBP2-29965
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reviewed by:
Alex Schmidt
WB Human 11/25/2014
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Summary

ApplicationWestern Blot
Sample TestedHeLa cells
SpeciesHuman

Blocking

Blocking Details4 % BSA in TBST

Primary Anitbody

Dilution RatioDiluted 1:1000 times

Secondary Antibody

Secondary DescriptionGoat Anti-Mouse IgG HRP Affinity Purified PAb (R&D Systems)
Secondary Manufacturer Cat#HAF007
Secondary ConcentrationDilution: 5,000 times

Details

Detection NotesOne-hour Western detection system (Genscript), 30 sec exposure

Comments

CommentsThis antibody gives a single band, unlike many others available. Also, it recognizes the recombinant protein.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EZH2/KMT6 Antibody (NBP2-29965) (0)

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Secondary Antibodies

 

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Alex Schmidt
11/25/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol EZH2
Entrez
Uniprot