EZH1 Antibody

Images

 
Western Blot: EZH1 Antibody [NBP1-76561] - Mouse lung tissue lysate with EZH1 antibody at (A) 1 and (B) 2 ug/mL
Immunocytochemistry/ Immunofluorescence: EZH1 Antibody [NBP1-76561] - Immunofluorescence of EZH1 in human lung tissue with EZH1 antibody at 20 ug/mL.
Immunohistochemistry: EZH1 Antibody [NBP1-76561] - Immunohistochemistry of EZH1 in human lung tissue with EZH1 antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

EZH1 Antibody Summary

Immunogen
Antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EZH1. The immunogen is located within amino acids 180 - 230 of EZH1.
Specificity
Multiple isoforms of EZH1 are known to exist. EZH1 antibody is predicted to not cross-react with EZH2
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EZH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
Control
Lung Lysate (NB820-59663)
Control Peptide
EZH1 Peptide (NBP1-76561PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EZH1 Antibody

  • EC 2.1.1.43
  • enhancer of zeste homolog 1 (Drosophila)
  • Enhancer of zeste homolog 1
  • ENX-2
  • histone-lysine N-methyltransferase EZH1
  • KIAA0388enhancer of zeste (Drosophila) homolog 1
  • KMT6B

Background

EZH1 was initially identified as a homolog of the drosophila Enhancer of Zeste through transcription mapping of chromosome 17q21. Both EZH1 and the related protein EZH2 can form complexes with the noncanonical Polycomb repressive complex-2 (PRC2) and maintain repressive chromatin, but the PRC2-EZH1 complex mediates methylation of histone H3. Both EZH1 and EZH2 are thought to function in the maintenance of embryonic stem cell pluripotency and plasticity and recently have been shown to be essential for hair follicle homeostasis and wound repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on EZH1.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

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Bioinformatics

Gene Symbol EZH1
Entrez
Uniprot