Exosome component 5 Antibody

Western Blot: Exosome component 5 Antibody [NBP2-14952] - HeLa whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Exosome component 5 Antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Exosome component 5 Antibody [NBP2-14952] - Exosome component 5 antibody detects Exosome component 5 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Exosome component 5 protein stained by Exosome component 5 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: Exosome component 5 Antibody [NBP2-14952] - BT483 xenograft, using EXOSC5 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Exosome component 5 Antibody Summary

• Immunogen: Full length human Exosome component 5 Recombinant protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EXOSC5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Exosome component 5 Antibody

• CML28
• exosome complex exonuclease RRP46
• exosome component 5Chronic myelogenous leukemia tumor antigen 28
• exosome component Rrp46
• hRrp46p
• MGC12901
• p12BMGC111224
• RRP41B
• Rrp46p
• RRP46Ribosomal RNA-processing protein 46

Background

Component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3'-untranslated regions. Required for the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA. Has a 3'-5' exonuclease activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EXOSC5