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Exosome component 4 Antibody

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Western Blot: Exosome component 4 Antibody [NBP3-03385] - Analysis of extracts of various cell lines, using Exosome component 4 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western Blot: Exosome component 4 Antibody [NBP3-03385] - Analysis of extracts of various cell lines, using Exosome component 4 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Exosome component 4 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human Exosome component 4 (NP_061910.1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EXOSC4
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.01% Thimerosal
Purity
Affinity purified

Alternate Names for Exosome component 4 Antibody

  • exosome complex exonuclease RRP41
  • exosome component 4Rrp41p
  • exosome component Rrp41
  • FLJ20591
  • p12ARibosomal RNA-processing protein 41
  • RRP41A
  • RRP41Ski6p
  • SKI6hRrp41p

Background

EXOSC4 is a component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAscontaining AU-rich elements (AREs) within their 3'-untranslated regions. Required for the 3'-processing of the 7Spre-RNA to the mature 5.8S rRNA. Has

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: RRP4 Antibody [NBP2-97759] - Immunochemical staining of human RRP4 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: RRP4 Antibody [NBP2-97759] - RRP4 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-RRP4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RRP4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 33 kDa
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Western Blot: Exosome component 10 Antibody [NBP1-32870] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EXOSC10 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of EXOSC 10 protein by 2. 5 ug of EXOSC 10 antibody 7. 5% SDS-PAGE gel.
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Western Blot: RHBDD1 Antibody [NBP1-85092] - Analysis in human cell line K562.
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Immunocytochemistry/Immunofluorescence: SKIV2L Antibody [NBP2-47274] - Staining of human cell line HeLa shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SKIV2L Antibody [NBP2-47274] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
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Flow Cytometry: Exosome component 7 Antibody (1G8) [NBP2-01732] - Analysis of Jurkat cells, using anti-Exosome component 7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Exosome component 7 Antibody (1G8) [NBP2-01732] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Exosome component 7 antibody, and then analyzed by flow cytometry.
Immunocytochemistry/Immunofluorescence: Exosome component 7 Antibody (1G8) [NBP2-01732] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Exosome component 7.
Species: Ca, Hu, Pm, Mu, Pm, Rt
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Western Blot: DCP1B Antibody [NBP1-82018] - Analysis in human cell line NTERA-2.
Immunohistochemistry-Paraffin: DCP1B Antibody [NBP1-82018] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunocytochemistry/Immunofluorescence: DCP1B Antibody [NBP1-82018] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
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Western Blot: Exosome component 8 Antibody [NBP2-47466] - Analysis in control (vector only transfected HEK293T lysate) and EXOSC8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: Exosome component 8 Antibody [NBP2-47466] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.
Immunohistochemistry-Paraffin: Exosome component 8 Antibody [NBP2-47466] - Staining of human fallopian tube shows cytoplasmic positivity in glandular cells.
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Western Blot: Exosome component 6 Antibody [NBP1-57508] - Jurkat cell lysate, Antibody Titration: 5.0ug/ml
Immunohistochemistry-Paraffin: Exosome component 6 Antibody [NBP1-57508] - Human Liver Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Hepatocytes (indicated with arrows) 400X magnification.
Immunohistochemistry-Paraffin: Exosome component 6 Antibody [NBP1-57508] - Human Muscle Tissue, Skeletal muscle cells (Indicated with Arrows) 4-8ug/ml.
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Immunocytochemistry/Immunofluorescence: DCP2 Antibody [NBP2-16109] - Zebrafish blastula cells expressing GFP-Rbpms2 fusions. Wild-type GFP-Rbpms2b localizes near the nucleus (top row), is apparently associated with the centrosome/spindle in some cells. (Top row) A subset of GFP-Rbpms2b positive granules are positive for the p-body marker Dcp2. (Second row) GFP-Rbpms2bsa9329 localization to the centrosome/spindle but not granules. (Third rod) GFP-Rbpms2bae32 and (Final row) GFP-Rbpms2aae30 localization. Insets show magnified views of the highlighted cells. Images are representative slices from Z-stacks of sphere stage embryos viewed from the animal pole. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pgen.1007489) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: DCP2 Antibody [NBP2-16109] - Mouse testis. DCP2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Immunocytochemistry/Immunofluorescence: Rpn2 Antibody [NBP2-38323] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & actin filaments.
Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human cerebral cortex, kidney, liver and testis using Anti-PSMD1 antibody NBP2-38323 (A) shows similar protein distribution across tissues to independent antibody NBP2-38324 (B).
Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human testis.
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Exosome component
4 Antibody
NBP3-03385
Exosome component 4 Antibody
Species: Hu, Rt
Applications: WB, IHC, IHC-P

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Bioinformatics

Gene Symbol EXOSC4