Eukaryotic translation initiation factor 5B Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Eukaryotic translation initiation factor 5B Antibody [NBP2-55202] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.

• Reactivity: Hu
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Eukaryotic translation initiation factor 5B Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Eukaryotic translation initiation factor 5B Antibody - BSA Free (NBP2-55202) is a polyclonal antibody validated for use in ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: EGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKRKKIPQQLESKEVSESMELCAAVEVMEQGVPEKE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF5B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
• Application Notes: Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Eukaryotic translation initiation factor 5B Recombinant Protein Antigen (NBP2-55202PEP)

Reactivity Notes

Mouse 81%, Rat 81%

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Eukaryotic translation initiation factor 5B Antibody - BSA Free

• DKFZp434I036
• eukaryotic translation initiation factor 5B
• FLJ10524
• IF2Translation initiation factor IF-2
• KIAA0741eIF-5B
• translation initiation factor IF2

Background

Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF5B