ETV2/ER71 Antibody

• Reactivity: Hu
• Applications: ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ETV2/ER71 Antibody Summary

• Description: Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.
• Immunogen: Peptide with sequence RCPKTNHRGPIQ corresponding to internal region according to NP_055024.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ETV2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
• Application Notes: WB: Preliminary experiments gave an approx. 24 kDa band in human testis lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 36.6 kDa band according to NP_055024.2. The 24 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ETV2/ER71 Antibody

• ER71
• ER71MGC129835
• ETS translocation variant 2
• ets variant 2
• Ets-related protein 71
• ETSRP71
• ETSRP71ets variant gene 2
• ETV2
• MGC129834

Background

ETV2 binds to DNA sequences containing the consensus pentanucleotide 5'-CGGA[AT]-3'

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ETV2/ER71 Antibody (NB100-93393)(1)

Publication using NB100-93393

• Knebel J, De Haro L, Janknecht R. Repression of transcription by TSGA/Jmjd1a, a novel interaction partner of the ETS protein ER71. J Cell Biochem 2006-09-01 [PMID: 16619273]

Bioinformatics

• Gene Symbol: ETV2
• Entrez:
  • 2116(Human)
• Uniprot:
  • B9EIN1(Human)