Ets-1 Antibody (8A8)

Images

 
Western Blot: Ets-1 Antibody (8A8) [NBP2-22169] - Western blot analysis using ETS1 mouse mAb against Jurkat (1), HepG2 (2) and ETS1-hIgGFc transfected HEK293 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: Ets-1 Antibody (8A8) [NBP2-22169] - Immunofluorescence analysis of NIH/3T3 cells using anti-ETS1 mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: Ets-1 Antibody (8A8) [NBP2-22169] - Immunohistochemical analysis of paraffin-embedded human Colon, Adipocytes tissues using anti-ETS1 mouse mAb
Flow Cytometry: Ets-1 Antibody (8A8) [NBP2-22169] - Flow cytometric analysis of Jurkat cells using anti-ETS1 mAb (green) and negative control (purple).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
8A8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Ets-1 Antibody (8A8) Summary

Immunogen
Purified recombinant fragment of human ETS1 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ETS1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
50.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Ets-1 Antibody (8A8)

  • Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1
  • ets protein
  • Ets1
  • Ets-1
  • EWSR2
  • FLJ10768
  • p54
  • Protein C-Ets-1
  • v-ets avian erythroblastosis virus E2 oncogene homolog 1
  • v-ets avian erythroblastosis virus E26 oncogene homolog 1
  • v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
  • v-ets

Background

The ETS1 is a transcription factor known to regulate expression of a number of genes involved in extracellular matrix remodeling. ETS1 is absent from normal gastric epithelium but is expressed in approximately 60% of gastric carcinomas and oral squamous cell carcinomas with a significant correlation to the tumor stage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Ets-1 Antibody (NBP2-22169)

Discover related pathways, diseases and genes to Ets-1 Antibody (NBP2-22169). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Ets-1.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

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Bioinformatics

Gene Symbol ETS1
Entrez