ETFB Antibody

Western Blot: ETFB Antibody [NBP1-49844] - Analysis of ETFB (aa 231-243) in Mouse Liver lysate (35ug protein in RIPA buffer) using NBP1-49844 at 0.01 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ETFB Antibody Summary

• Immunogen: Peptide with sequence C-PQRTAGVKVETTE corresponding to C-Terminus according to NP_001014763.1.
• Epitope: PQRTAGVKVETTE - C Terminus
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1). Amino acid numbering in the name above refers to NP_001976.1).
• Predicted Species: Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ETFB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: WB: Approx. 26 kDa band observed in human, mouse and rat liver lysates (calculated MW of 27.8 kDa band according to NP_001976.1). IHC-P: Human liver shows mitochondrial staining in hepatocytes.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ETFB Antibody

• beta-ETF
• electron transfer flavoprotein beta subunit
• electron transfer flavoprotein beta-subunit
• electron transfer flavoprotein subunit beta
• electron transfer flavoprotein, beta polypeptide
• electron-transfer-flavoprotein, beta polypeptide
• electron-transferring-flavoprotein, beta polypeptide
• MADD

Background

This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ETFB Antibody (NBP1-49844)(1)

Publication using NBP1-49844

• Ohkuma A, Noguchi S, Sugie H et al. Clinical and genetic analysis of lipid storage myopathies. Muscle Nerve 2009-03-01 [PMID: 19208393]

Bioinformatics

• Gene Symbol: ETFB
• Entrez:
  • 2109(Human)
  • 110826(Mouse)
  • 292845(Rat)
• Uniprot:
  • NP_001976.1(Human)