ETFA Antibody

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Western Blot: ETFA Antibody [NBP1-49845] - Analysis of ETFA in Human Heart lysate (35ug protein in RIPA buffer) using NBP1-49845 at 0.1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

ETFA Antibody Summary

Immunogen
Peptide with sequence C-QVADYGIVADLFK corresponding to C-Terminus according to NP_001121188.1.
Epitope
QVADYGIVADLFK - C Terminus
Specificity
This antibody is expected to recognize both reported isoforms (NP_000117.1; NP_001121188.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ETFA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.05 - 0.2 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 30 kDa band observed in human, mouse and rat heart lysates (calculated MW of 30.0 kDa band according to NP_001121188.1).
Publications
Read Publication using NBP1-49845.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ETFA Antibody

  • alpha-ETF
  • electron transfer flavoprotein alpha-subunit
  • Electron transfer flavoprotein subunit alpha, mitochondrial
  • electron-transfer-flavoprotein, alpha polypeptide
  • EMA
  • GA2
  • glutaric aciduria II
  • MADD

Background

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ETFA Antibody (NBP1-49845)(1)


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Bioinformatics

Gene Symbol ETFA
Entrez
Uniprot