ESAM Antibody

Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ESAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2688) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ESAM at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human ESAM Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2688, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ESAM Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ESAM
  Gln30-Ala247
  Accession # Q96AP7
• Specificity: Detects human ESAM in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant mouse ESAM is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ESAM
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ESAM Antibody

• 2310008D05Rik
• endothelial cell adhesion molecule
• endothelial cell-selective adhesion molecule
• ESAM
• HUEL (C4orf1)-interacting protein
• LP4791 protein
• W117m

Background

Endothelial cell-selective adhesion molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules (1, 2). Human ESAM is synthesized as a 390 amino acid (aa) protein composed of a 29 aa signal peptide, a 216 aa extracellular region, a putative 26 aa transmembrane segment, and a 119 aa cytoplasmic domain. The extracellular region contains one V-type and one C2-type Ig domain and is involved in homophilic adhesion (1). In the cytoplasmic domain, there is a docking site for the multifunctional adaptor protein MAGI-1 (3). The extracellular region of human ESAM shows 90%, 74%, 69% and 67% aa identity with monkey, canine, mouse and rat extracellular ESAM, respectively. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes, and can be found associated with cell-to-cell junctions. Whether ESAM is restricted to a particular junctional type is not clear (1, 2). ESAM deficient mice have no defect in vascularization but do have reduced angiogenic potential. This may be due to a decreased migratory response to FGF-2 (4).

1. Hirata, K-I, et al. (2001) J. Biol. Chem. 276:16223.
2. Nasdala, I. et al. (2002) J. Biol. Chem. 277:16294.
3. Wegmann, F. et al. (2004) Exp. Cell Res. 300:121.
4. Ishida, T. et. al. (2003) J. Biol. Chem. 278:34598.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ESAM Antibody (AF2688)(2)

Publications using AF2688

• NR Hansmeier, IS Büschlen, RY Behncke, S Ulferts, R Bisoendial, R Hägerling 3D Visualization of Human Blood Vascular Networks Using Single-Domain Antibodies Directed against Endothelial Cell-Selective Adhesion Molecule (ESAM) International Journal of Molecular Sciences, 2022-04-15;23(8):. 2022-04-15 [PMID: 35457187] (IHC, Human)
• Roesli C, Mumprecht V, Neri D, Detmar M Identification of the surface-accessible, lineage-specific vascular proteome by two-dimensional peptide mapping. FASEB J., 2008-01-07;22(6):1933-44. 2008-01-07 [PMID: 18180333] (IHC-Fr, Human)

Bioinformatics

• Gene Symbol: ESAM
• Uniprot:
  • Q96AP7()