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AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox" target="_blank">
VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of vascular endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of vascular endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
HAF019). A specific band was detected for ESAM at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox" target="_blank">
MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B)." class="big_lightbox" target="_blank">
ESAM is a tight junction regulatory protein involved in cell adhesion and is expressed only on endothelial cells and activated platelets. It may mediate aggregation through a homophilic molecular interaction (By similarity). ESAM at endothelial tight junctions participates in the migration of neutrophils through the vessel walls and it may have a role in signaling mechanisms that trigger the opening of endothelial cell contacts. A correlation has been found between ESAM expression and lymph node metastasis.
![Flow Cytometry ESAM Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af2827_mouse-esam-affinity-purified-polyclonal-ab-flow-cytometry-18122024131854..jpg)
![Flow Cytometry ESAM Antibody (408519) [Unconjugated]](http://images.novusbio.com/images/antibody/ESAM_MAB4204_Flow_Cytometry_8437.jpg)
![Immunohistochemistry ESAM Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af2827_mouse-esam-affinity-purified-polyclonal-ab-immunohistochemistry-2211202412527..jpg)
![Immunohistochemistry ESAM Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af2827_mouse-esam-affinity-purified-polyclonal-ab-immunohistochemistry-2211202412614..jpg)
![Western Blot ESAM Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2827_mouse-esam-affinity-purified-polyclonal-ab-western-blot-121220259153618.jpg)
![Western Blot ESAM Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2827_mouse-esam-affinity-purified-polyclonal-ab-western-blot-12122025857590.jpg)
![Immunocytochemistry ESAM Antibody (408519) [Unconjugated]](http://images.novusbio.com/images/antibody/ESAM_MAB4204_Immunocytochemistry_10122.jpg)
