ESAM Antibody (408519) [Unconjugated]

ESAM was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human ESAM Monoclonal Antibody (Catalog # MAB4204) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conju­gated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human ESAM Monoclonal Antibody (Catalog # MAB4204, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 408519
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ESAM Antibody (408519) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ESAM
  Gln30-Ala247
  Accession # Q96AP7
• Specificity: Detects human ESAM in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse ESAM is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ESAM

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ESAM Antibody (408519) [Unconjugated]

• 2310008D05Rik
• endothelial cell adhesion molecule
• endothelial cell-selective adhesion molecule
• ESAM
• HUEL (C4orf1)-interacting protein
• LP4791 protein
• W117m

Background

Endothelial cell-selective adhesion molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules (1, 2). Human ESAM is synthesized as a 390 amino acid (aa) protein composed of a 29 aa signal peptide, a 216 aa extracellular region, a putative 26 aa transmembrane segment, and a 119 aa cytoplasmic domain. The extracellular region contains one V-type and one C2-type Ig domain and is involved in homophilic adhesion (1). In the cytoplasmic domain, there is a docking site for the multifunctional adaptor protein MAGI-1 (3). The extracellular region of human ESAM shows 90%, 74%, 69%, and 67% aa identity with monkey, canine, mouse, and rat extracellular ESAM, respectively. ESAM is expressed on endothelial cells, activated platelets, and megakaryocytes and can be found associated with cell-to-cell junctions. Whether ESAM is restricted to a particular junctional type is not clear (1, 2). ESAM deficient mice have no defect in vascularization but do have reduced angiogenic potential. This may be due to a decreased migratory response to FGF-2 (4).

1. Hirata, K-I. et al. (2001) J. Biol. Chem. 276:16223.
2. Nasdala, I. et al. (2002) J. Biol. Chem. 277:16294.
3. Wegmann, F. et al. (2004) Exp. Cell Res. 300:121.
4. Ishida, T. et. al. (2003) J. Biol. Chem. 278:34598.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ESAM
• Uniprot:
  • Q96AP7()