ERO1LB Antibody (M37-P5D11) - BSA Free

Western Blot: ERO1LB Antibody (M37-P5D11) [NBP2-50389] - Shows detection of a 62 kDa and 49 kDa band in a Jurkat cell lysate.

Immunohistochemistry-Paraffin: ERO1LB Antibody (M37-P5D11) [NBP2-50389] - Immunohistochemistry images are showing a stronger cytoplasmic staining of ERO1LB in (A) primary colorectal cancer compared to (B) normal colon mucosa using ERO1LB Antibody (M37-P5D11). IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).

Immunohistochemistry: ERO1LB Antibody (M37-P5D11) [NBP2-50389] - Staining of a cross section from breast cancer tissue.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: M37-P5D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ERO1LB Antibody (M37-P5D11) - BSA Free Summary

• Description: Novus Biologicals Mouse ERO1LB Antibody (M37-P5D11) - BSA Free (NBP2-50389) is a monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide - LAPSRGEDDG
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ERO1B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100 - 1:2000
• Application Notes: Positive control(s): WB - Jurkat whole cell lysate, IHC - formalin-fixed, paraffin-embedded human breast cancer.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ERO1LB Antibody (M37-P5D11) - BSA Free

• DKFZp779C1042
• DKFZp779I0141
• EC 1.8.4
• EC 1.8.4.-
• Endoplasmic oxidoreductin-1-like protein B
• ERO1-L(beta)
• ERO1-L-beta
• ERO1-like beta (S. cerevisiae)
• ERO1-like protein beta
• FLJ11003
• oxidoreductin-1-L-beta

Background

Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the being a source of oxidative stress. Required for the folding of cell, thereby being a source of oxidative stress

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ERO1B