ERManI Antibody - BSA Free

Immunohistochemistry-Paraffin: ERManI Antibody [NBP2-14216] - Staining of human duodenum shows moderate cytoplasmic positivity with a granular pattern in glandular cells.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ERManI Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to the amino acids: METGLSPEIVHFNLYPQPGRRDVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVPSGGYSSINNVQDPQKP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAN1B1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  ERManI Protein (NBP2-14216PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (84%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ERManI Antibody - BSA Free

• alpha 1,2-mannosidase
• EC 3.2.1.113
• endoplasmic reticulum alpha-mannosidase 1
• endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase 1
• endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
• ER alpha 1,2-mannosidase
• ER alpha-1,2-mannosidase
• ER ManI
• ER mannosidase 1
• ERMan1
• Man9GlcNAc2-specific processing alpha-mannosidase
• Man9GlcNAc2-specific-processing alpha-mannosidase
• MANA-ER
• Mannosidase alpha class 1B member 1
• mannosidase, alpha, class 1B, member 1
• MRT15
• RP11-350O14.2

Background

ERManI (MAN1B1) catalyzes the first mannose-trimming step in maturation of Asn-linked oligosaccharides. It trims a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2), specifically converting it to Man8GlcNAc isomer B. ERManI may be involved in glycoprotein quality control since it is important to target misfolded glycoproteins for degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAN1B1