ERK1/2 Antibody (SB46b) - Azide and BSA Free

Western Blot: ERK1/2 Antibody (SB46b) [NBP1-28627] - Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Erk1/2-BIOT (SB Cat. No. 12080-08). Proteins were visualized using Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05) secondary antibody and chemiluminescent detection.

Western Blot: ERK1/2 Antibody (SB46b) [NBP1-28627] - 5ug/mL Human PBMC and Spleen Lysate was stained with Mouse Anti-Erk 1/2. Goat anti-Mouse IgG2a was used to develop.

Western Blot: ERK1/2 Antibody (SB46b) [NBP1-28627] - Jurkat (Lane 1), HEp-2 (Lane 2), and 3T3 (Lane 3) Lysate was stained with Mouse Anti-Erk 1/2-BIOT. Mouse Anti-Biotin-HRP was used to develop.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: SB46b
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

ERK1/2 Antibody (SB46b) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ERK1/2 Antibody (SB46b) - Azide and BSA Free (NBP1-28627) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant Erk1 and Erk 2
• Specificity: Reacts with human Erk 1 and human Erk2
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPK3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1-2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for ERK1/2 Antibody (SB46b) - Azide and BSA Free

• EC 2.7.11
• ERK-1
• ERK1p44-MAPK
• ERT2
• Extracellular signal-regulated kinase 1
• extracellular signal-related kinase 1
• HS44KDAP
• HUMKER1A
• Insulin-stimulated MAP2 kinase
• MAP kinase 1
• MAP kinase 3
• MAP kinase isoform p44
• MAPK 1
• MAPK 3
• MGC20180
• Microtubule-associated protein 2 kinase
• Mitogen-activated protein kinase 1
• mitogen-activated protein kinase 3
• p44erk1
• p44-ERK1
• p44mapk
• PRKM3EC 2.7.11.24

Background

Erk1 and Erk2 are closely related mitogen activated protein (MAP) kinases which are activated by many growth factors, mitogens and differentiation-promoting agents via a protein kinase cascade. Also known as extracellular signal-regulated kinase 1 and 2, p44/p42 MAP kinases, microtubule-associated protein-2 kinases, myelin basic protein (MBP) kinases or EGF receptor T669 (Ert) kinases (2-5), Erk1 and Erk2 are ubiquitous and abundant, although their relative abundance in specific tissues may vary. The two kinases are nearly 85% identical and have higher identity in the core regions involved in substrate binding 5. Erk1 and Erk2 are activated approximately 1000-fold by phosphorylation within a Thr-Glu-Tyr motif in the activation loop on both threonine and tyrosine residues by Mek1 and Mek2 (5,6). Both sites must be phosphorylated for maximum activity (2-5). These kinases in turn phosphorylate a variety of different substrates. Erk1 and Erk2 are found in the cytoplasm and are translocated to the nucleus upon activation. Erk1 and Erk2 target membrane proteins, cytosolic proteins, such as downstream kinases, and cytoskeletal proteins and nuclear proteins, such as transcription factors. Many of these substrates are important regulatory proteins. Erk1 and Erk2 represent proximal kinases in the classical kinase pathway which links growth and differentiation signals at the cell surface (through tyrosine kinases) with transcription in the nucleus (2-4). The SB46b monoclonal antibody precipitates approx. 42 kDa and approx. 44 kDa bands, corresponding to Erk1 and Erk2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK3