eRF1 Antibody (2H4) - Azide and BSA Free

Western Blot: eRF1 Antibody (2H4) [H00002107-M02] - Analysis of ETF1 expression in transfected 293T cell line by ETF1 monoclonal antibody (M02), clone 2H4.Lane 1: ETF1 transfected lysate (Predicted MW: 49 KDa).Lane 2: Non-transfected lysate.

Western Blot: eRF1 Antibody (2H4) [H00002107-M02] - ETF1 monoclonal antibody (M02), clone 2H4. Analysis of ETF1 expression in HepG2.

Sandwich ELISA: eRF1 Antibody (2H4) [H00002107-M02] - Detection limit for recombinant GST tagged ETF1 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 2H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

eRF1 Antibody (2H4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ETF1 (NP_004721.1, 338 a.a. ~ 437 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TEEEKILYLTPEQEKDKSHFTDKETGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGMEYQGGDDEFFDLDDY
• Specificity: ETF1 - eukaryotic translation termination factor 1 (2H4)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ETF1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for eRF1 Antibody (2H4) - Azide and BSA Free

• D5S1995
• ERF1eRF1
• eukaryotic peptide chain release factor subunit 1
• Eukaryotic release factor 1
• eukaryotic translation termination factor 1
• polypeptide chain release factor 1
• Protein Cl1
• RF1ERF
• sup45 (yeast omnipotent suppressor 45) homolog-like 1
• SUP45L1
• TB3-1MGC111066

Background

Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ETF1
• Entrez:
  • 2107(Human)
• Uniprot:
  • NP_004721(Human)