ERCC6L Antibody - Azide and BSA Free

Western Blot: ERCC6L Antibody [H00054821-B01P] - Analysis of ERCC6L expression in HeLa.

Western Blot: ERCC6L Antibody [H00054821-B01P] - Analysis of ERCC6L expression in transfected 293T cell line by ERCC6L polyclonal antibody. Lane 1: ERCC6L transfected lysate(46.09 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ERCC6L Antibody - Azide and BSA Free Summary

• Immunogen: ERCC6L (AAH08808.1, 1 a.a. - 419 a.a.) full-length human protein. MEKSFATKNEAVQKETLQEGPKQEALQEDPLESFNYVLSKSTKADIGPNLDQLKDDEILRHCNPWPIISITNESQNAESNVSIIEIADDLSASHSALQDAQASEAKLEEEPSASSPQYACDFNLFLEDSADNRQNFSSQSLEHVEKENSLCGSAPNSRAGFVHSKTCLSWEFSEKDDEPEEVVVKAKIRSKARRIVSDGEDEDDSFKDTSSINPFNTSLFQFSSVKQFDASTPKNDISPPGRFFSSQIPSSVNKSMNSRRSLASRRSLINMVLDHVEDMEERLDDSSEAKGPEDYPEEGVEESSGEASKYTEEDPSGETLSSENKSSWLMTSKPSALAQETSLGAPEPLSGEQLVGSPQDKAAEATNDYETLVKRGKELKECGKIQEALNCLVKALDIKSADPEVMLLTLSLYKQLNNN
• Specificity: ERCC6L - excision repair cross-complementing rodent repair deficiency, complementation group 6-like,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: ERCC6L
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. Use in ICC/IF was reported in the scientific literature (PMID: 23811685).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ERCC6L Antibody - Azide and BSA Free

• ATP-dependent helicase ERCC6-like
• DNA excision repair protein ERCC-6-like
• EC 3.6.1
• EC 3.6.4.12
• excision repair cross-complementing rodent repair deficiency, complementationgroup 6-like
• excision repair protein ERCC6-like
• FLJ20105
• MGC131695
• PICHexcision repair cross-complementing rodent repair deficiency complementationgroup 6 - like
• PLK1-interacting checkpoint helicase
• SNF2/RAD54 family protein
• Tumor antigen BJ-HCC-15

Background

DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERCC6L Antibody (H00054821-B01P)(3)

Publications using H00054821-B01P

• Tiwari A, Addis Jones O, Chan KL. et al. 53BP1 can limit sister-chromatid rupture and rearrangements driven by a distinct ultrafine DNA bridging-breakage process. Nat Commun 2018-02-14 [PMID: 29445165]
• Beishline K, Vladimirova O, Tutton S et al. CTCF driven TERRA transcription facilitates completion of telomere DNA replication. Nat Commun 2017-12-13 [PMID: 29235471]
• Nera B, Huang HS, Lai T, Xu L. Elevated levels of TRF2 induce telomeric ultrafine anaphase bridges and rapid telomere deletions. Nat Commun 2015-12-07 [PMID: 26640040]

Bioinformatics

• Gene Symbol: ERCC6L
• Entrez:
  • 54821(Human)
• Uniprot:
  • AAH08808.1(Human)