ERCC1 Antibody (CL1284) - Azide and BSA Free

Staining of human breast cancer shows moderate nuclear positivity in tumor cells.

Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.

Staining of human prostate cancer shows moderate to strong nuclear positivity in tumor cells.

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ERCC1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Staining of human tonsil shows moderate nuclear positivity in lymphoid cells.

Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clone: CL1284
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ERCC1 Antibody (CL1284) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ERCC1 Antibody (CL1284) - Azide and BSA Free (NBP2-34480) is a monoclonal antibody validated for use in IHC, WB and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P07992, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ERCC1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Simple Western 1:20
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. See Simple Western Antibody Database for Simple Western validation: Tested in RT4, separated by Size, antibody dilution of 1:20, apparent MW was 46 kDa

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for ERCC1 Antibody (CL1284) - Azide and BSA Free

• COFS4
• DNA excision repair protein ERCC-1
• ERCC1
• excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence)
• RAD10
• UV20

Background

The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1 XPF complex makes the 5'incision. Elevated levels of ERCC1 have also been reported in Cisplatin resistant cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ERCC1