ErbB2/Her2 Antibody (HRB2/258) [Alexa Fluor® 647]

• Reactivity: Hu, Mu, Rt
• Applications: ELISA, Flow, ICC/IF, CyTOF-ready
• Clone: HRB2/258
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

ErbB2/Her2 Antibody (HRB2/258) [Alexa Fluor® 647] Summary

• Immunogen: Recombinant extracellular domain of human ErbB2/Her2 protein (Uniprot: IDP04626)
• Localization: Cell Surface
• Specificity: Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This monoclonal antibody is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. C-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ERBB2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ErbB2/Her2 Antibody (HRB2/258) [Alexa Fluor® 647]

• CD340 antigen
• CD340
• c-erb B2/neu protein
• EC 2.7.10
• EGFR2
• ErbB2
• HER2
• HER-2
• HER2EC 2.7.10.1
• herstatin
• Metastatic lymph node gene 19 protein
• MLN 19
• MLN19
• Neu Oncogene
• NEUHER-2/neu
• neuroblastoma/glioblastoma derived oncogene homolog
• NGL
• NGLTKR1
• p185erbB2
• Proto-oncogene c-ErbB-2
• Proto-oncogene Neu
• receptor tyrosine-protein kinase erbB-2
• TKR1
• Tyrosine kinase-type cell surface receptor HER2
• v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog)
• v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Background

ErbB2/HER2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for ErbB2/Her2 Antibody (NBP2-34641AF647). (Showing 1 - 4 of 4 FAQ).

1. I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
   • A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22
2. I'm interested in purchasing a monoclonal anti-HER2(human) primary antibody, conjugated to Alexa Fluor 647 (or possibly 488). I was curious whether your product NBP2-34641AF647 binds to the extracellular or intracellular domain of HER2? We are interested
   • NBP2-34641AF647 and 488 is made to a recombinant fragment corresponding to the extracellular domain of the human HER2 protein. The epitope is localized in the extracellular domain.
3. I was curious what 100 tests corresponds to, concentration wise?
   • 100 tests corresponds to 100ug as 1ug/test. The base product of this antibody (NBP2-34641) is supplied at 1mg/ml, so the vial will have 100ul of volume. The vial of conjugated antibody will contain at least 100ug of antibody.
4. I am curious whether the epitope of the antibody NBP2-34641AF647 is located intra- or extracellularly? I would like to use this antibody to sort cells and thus need to avoid any permeabilization or fixation steps.
   • The immunogen used to generate this antibody is a recombinant extracellular domain of human HER-2. Unfortunately, I do not have access to the exact immunogen sequence as that information is proprietary.

Bioinformatics

• Gene Symbol: ERBB2