ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human ErbB2/Her2 Herstatin Isoform Monoclonal Antibody (Catalog # MAB11291) at 25 µg/mL overnight at 4 °C. Tissue ...read more
ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human ErbB2/Her2 Herstatin Isoform Monoclonal Antibody (Catalog # MAB11291) at 8 µg/mL ...read more
Herstatin is a secreted 68 kDa glycoprotein encoded by an alternate transcript of the HER-2/neu (erbB-2) gene containing the N-terminal 304 amino acids (aa) of subdomain I and II, and 79 intron-encoded aa at the C-terminus. It is an endogenous inhibitor of the EGF receptor family that disrupts receptor interactions and inhibits growth of tumor cells that overexpress HER-2.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Used Scytek IHC kit: H2O2 block ADA008 (5 min) and Superblock AAA008 (4 min) room temp.
Used Novus mAb11291 at 1:20 dilution in TBS-Tween for one hour at room temp.
Scytek polyvalent HRP polymer from kit
Secondary Manufacturer Cat#
Negcontrol received isotope control antibody. DAB from Scytek Kit: 2X5 mins incubation. Images were taken with a Spot camera.
10 min. fixation in cytospin solution with methanol/acetone/glycerol, spun onto slides, dried, then incubated w/TBS-Tween
PBS washes 3X between each step.
FAQs for ErbB2/Her2 Antibody (MAB11291). (Showing 1 - 1 of 1 FAQ).
I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22
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