ErbB2/Her2 Antibody (416711)

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ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human ErbB2/Her2 Herstatin Isoform Monoclonal Antibody (Catalog # MAB11291) at 25 µg/mL overnight at 4 °C. Tissue ...read more
ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human ErbB2/Her2 Herstatin Isoform Monoclonal Antibody (Catalog # MAB11291) at 8 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
416711
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

ErbB2/Her2 Antibody (416711) Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ErbB2/Her2 Herstatin Isoform
Thr23-Gly419
Accession # AAD56009
Specificity
Detects human ErbB2/Her2 Herstatin Isoform in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) EGFR or rhErbB3 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ERBB2
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
Control
ErbB2/Her2 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
MAB11291 in the following application:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ErbB2/Her2 Antibody (416711)

  • CD340 antigen
  • CD340
  • c-erb B2/neu protein
  • EC 2.7.10
  • EGFR2
  • ErbB2
  • HER2
  • HER-2
  • HER2EC 2.7.10.1
  • herstatin
  • Metastatic lymph node gene 19 protein
  • MLN 19
  • MLN19
  • Neu Oncogene
  • NEUHER-2/neu
  • neuroblastoma/glioblastoma derived oncogene homolog
  • NGL
  • NGLTKR1
  • p185erbB2
  • Proto-oncogene c-ErbB-2
  • Proto-oncogene Neu
  • receptor tyrosine-protein kinase erbB-2
  • TKR1
  • Tyrosine kinase-type cell surface receptor HER2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog)
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Background

Herstatin is a secreted 68 kDa glycoprotein encoded by an alternate transcript of the HER-2/neu (erbB-2) gene containing the N-terminal 304 amino acids (aa) of subdomain I and II, and 79 intron-encoded aa at the C-terminus. It is an endogenous inhibitor of the EGF receptor family that disrupts receptor interactions and inhibits growth of tumor cells that overexpress HER-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ErbB2/Her2 Antibody (MAB11291) (0)

There are no publications for ErbB2/Her2 Antibody (MAB11291).
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Review for ErbB2/Her2 Antibody (MAB11291) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB11291:
Filter by Applications
ICC
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry ErbB2/Her2 MAB11291
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reviewed by:
Gail Seigel
ICC Human 06/08/2015
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman BT474 Breast Cancer Cells
SpeciesHuman

Blocking

Blocking DetailsUsed Scytek IHC kit: H2O2 block ADA008 (5 min) and Superblock AAA008 (4 min) room temp.

Primary Anitbody

Dilution RatioUsed Novus mAb11291 at 1:20 dilution in TBS-Tween for one hour at room temp.

Secondary Antibody

Secondary DescriptionScytek polyvalent HRP polymer from kit
Secondary Manufacturer Cat#ABZ008
Secondary ConcentrationReady-to-Use

Details

Detection NotesNegcontrol received isotope control antibody. DAB from Scytek Kit: 2X5 mins incubation. Images were taken with a Spot camera.
Fixation Details10 min. fixation in cytospin solution with methanol/acetone/glycerol, spun onto slides, dried, then incubated w/TBS-Tween
Wash DescriptionPBS washes 3X between each step.

FAQs for ErbB2/Her2 Antibody (MAB11291). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
    • A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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5
1
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1
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Gail Seigel
06/08/2015
Application: ICC
Species: Human

Bioinformatics

Gene Symbol ERBB2
Uniprot