epithelial Sodium Channel gamma Antibody

Immunohistochemistry-Paraffin: epithelial Sodium Channel gamma Antibody [NBP1-06075] - (4ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

epithelial Sodium Channel gamma Antibody Summary

• Description: Novus Biologicals Goat epithelial Sodium Channel gamma Antibody (NBP1-06075) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-epithelial Sodium Channel gamma Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SNQLTDTQMLDE corresponding to C-Terminus according to NP_001030.2.
• Epitope: C-SNQLTDTQMLDE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SCNN1G
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 4 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 ug/ml
• Application Notes: Western blot: Preliminary experiments gave an approx 140kDa band in Human Breast cancer and Colon lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 74.3kDa according to NP_001030.2. The 140kDa band was successfully blocked by incubation with the immunizing peptide.
  IHC: Paraffin embedded Human Colon.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for epithelial Sodium Channel gamma Antibody

• amiloride-sensitive epithelial sodium channel gamma subunit
• amiloride-sensitive sodium channel gamma-subunit
• BESC3
• ENaC gamma subunit
• ENaCG
• ENaCgamma
• Epithelial Na(+) channel subunit gamma
• gamma-ENaC
• gamma-NaCH
• Nonvoltage-gated sodium channel 1 subunit gamma
• PHA1
• SCNEGamiloride-sensitive sodium channel subunit gamma
• sodium channel, nonvoltage-gated 1, gamma

Background

Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the gamma subunit, and mutations in this gene have been associated with Liddle syndrome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for epithelial Sodium Channel gamma Antibody (NBP1-06075)(1)

Publication using NBP1-06075

• Boyd C, Naray-Fejes-Toth A. Steroid-mediated regulation of the epithelial sodium channel subunits in mammary epithelial cells. Endocrinology 2007-08-01 [PMID: 17510235]

Bioinformatics

• Gene Symbol: SCNN1G
• Entrez:
  • 6340(Human)
• Uniprot:
  • NP_001030.2(Human)