epithelial Sodium Channel gamma Antibody (5c2) - BSA Free

Immunohistochemistry-Paraffin: epithelial Sodium Channel gamma Antibody (5c2) - BSA Free [NBP2-41373] - Analysis using the Biotin conjugate of NBP2-41373. Staining of Human kidney tissue. Courtesy of Per Svenningsen, PhD.

Sandwich ELISA: epithelial Sodium Channel gamma Antibody (5c2) - BSA Free [NBP2-41373] - Analysis using the Biotin conjugate of NBP2-41373. The calibration curveof a sandwich assay using NBP2-41371 as the capture antibody and NBP2-41372B as the biotinylated detection antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 5c2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

epithelial Sodium Channel gamma Antibody (5c2) - BSA Free Summary

• Description: Novus Biologicals Mouse epithelial Sodium Channel gamma Antibody (5c2) - BSA Free (NBP2-41373) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The inhibitory peptide from the human yENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to amino acid residue 139-160 of human yENaC subunit.
• Epitope: The epitope is on the inhibitory tract of human yENaC.
• Specificity: Antibody is specific for the inhibitory tract of human gENaC.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SCNN1G
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: A sandwich ELISA can be made using NBP2-41371 (1-4 ug/ml) as the capture antibody and biotinylated NBP2-41373 (0.05 - 0.2 ug/ml) as the detection antibody in order to detect the peptide from the inhibitory tract (AA 138-131). WB: NBP2-41373 was used in Western blot. (1) IHC: NBP2-41373 was used in immunohistochemistry. (1) IF: NBP2-41373 was used in immunofluorescence. (1)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for epithelial Sodium Channel gamma Antibody (5c2) - BSA Free

• amiloride-sensitive epithelial sodium channel gamma subunit
• amiloride-sensitive sodium channel gamma-subunit
• BESC3
• ENaC gamma subunit
• ENaCG
• ENaCgamma
• Epithelial Na(+) channel subunit gamma
• gamma-ENaC
• gamma-NaCH
• Nonvoltage-gated sodium channel 1 subunit gamma
• PHA1
• SCNEGamiloride-sensitive sodium channel subunit gamma
• sodium channel, nonvoltage-gated 1, gamma

Background

Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the gamma subunit, and mutations in this gene have been associated with Liddle syndrome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCNN1G