Epiregulin Antibody (183629) [Alexa Fluor® 350]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
183629
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 350
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Epiregulin Antibody (183629) [Alexa Fluor® 350] Summary

Immunogen
E. coli-derived recombinant human Epiregulin
Val63-Leu108
Accession # O14944
Specificity
Detects human Epiregulin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Betacellulin, rhEGF, recombinant mouse Epiregulin, or rhHB-EGF is observed.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
EREG
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Epiregulin Antibody (183629) [Alexa Fluor® 350]

  • Epiregulin
  • ER
  • EREG
  • proepiregulin

Background

Epiregulin is a member of the EGF family of growth factors which includes, among others, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, amphiregulin (ARG), HB (heparin-binding)-EGF, betacellulin, and the various heregulins. All EGF family members are synthesized as transmembrane precursors and are converted to soluble forms by proteolytic cleavage. Epiregulin was originally purified from the mouse fibroblast-derived tumor cell line NIH3T3/T7 (1). The human epiregulin cDNA encodes a 169 amino acid (aa) residues transmembrane precursor with a 29 aa signal peptide, a 21 aa transmembrane domain and a 21 aa cytoplasmic domain. The putative soluble mature Epiregulin comprising the EGF-like domain (aa residues 64‑104) is formed by proteolytic removal of the propeptide regions (2). There is 85% aa sequence homology between human and mouse epiregulins. Epiregulin is expressed primarily in the placenta and macrophages (3). High level expression has also been detected in various carcinomas. Epiregulin specifically binds EGFR (ErbB1) and ErbB4 but not ErbB2 and ErbB3. It activates the homodimers of both ErbB1 and ErbB4. In addition, epiregulin can also activate all possible heteromeric combinations of the four ErbB family members (4). Epiregulin stimulates the proliferation of fibroblasts, smooth muscle cells and hepatocytes. It has been shown to be an autocrine growth factor for epidermal keratinocytes as well as mesangial cells (5, 6). Epiregulin has also been shown to inhibit growth of several epithelial tumor cells. In addition, Epiregulin has been implicated in the implantation process during pregnancy (7).

  1. Toyoda, H. et al. (1995) J. Biol. Chem. 270:7495.
  2. Toyoda, H. et al. (1997) Biochem. J. 326:69.
  3. Komurasaki, T. et al. (1997) Oncogene 15:2841.
  4. Shelly, M. et al. (1998) J. Biol. Chem. 273:10496.
  5. Shirakata, Y. et al. (2000) J. Biol. Chem. 275:5748.
  6. Mishre, R. et al. (2002) Am. J. Physiol. Renal. Physiol. 283:F1151.
  7. Das, S.K. et al. (1997) Dev. Biol. 190:178.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol EREG
Uniprot