Epigen Antibody (187204)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA(Cap)
Clone
187204
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Epigen Antibody (187204) Summary

Immunogen
E. coli-derived recombinant mouse Epigen
Leu53-Ala103
Accession # Q924X1.1
Specificity
Detects mouse Epigen in ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
Epgn
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Mouse Epigen Antibody (Catalog # MAB11271)
ELISA Detection: Mouse Epigen Biotinylated Antibody (Catalog # BAF1127)
Standard: Recombinant Mouse Epigen (Catalog # 1127-EP)
Publications
Read Publication using
MAB11271 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

U.S. Patent No. 6,150,502 used under license from Genesis Research and Development Corporation Limited



This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Epigen Antibody (187204)

  • ALGV3072
  • EPG
  • EPGN
  • Epigen
  • PRO9904

Background

Epigen (EPGN) is an epithelial mitogen that belongs to the EGF superfamily (1-3). Mouse Epigen cDNA encodes a 152 amino acid (aa) residue type I transmembrane precursor protein with a 23 aa putative signal peptide, an 86 aa extracellular domain, a 21 aa transmembrane domain and a 22 aa cytoplasmic domain (2, 3). The extracellular domain (aa 24-109) contains a pattern of six cysteines typical of EGF family proteins (1-3). Epigen is among several EGF family proteins that can undergo metalloproteinase cleavage to generate a soluble form (3, 4). Cleavage of Epigen by TACE/ADAM17 has been demonstrated (4). The mature, shed form of mouse Epigen (aa 53-103) shares 92%, 96% and 90% aa sequence identity with human, rat and equine Epigen, respectively, and more than 40% aa identity with corresponding regions of TGF-alpha and epiregulin (2). Epigen mRNA is found in many tissues, but it is mainly expressed in actively proliferating cells within these tissues. For example, Epigen in skin is found mainly in the proliferating root sheath of hair follicles, and transgenic overexpression in the skin causes hyperplasia in sebaceous glands (3, 5). Epigen is also expressed developmentally and by invasive adenocarcinomas of the breast and prostate (3). Epigen is strongly mitogenic for epithelial cells and fibroblasts, despite its relatively weak affinity for its main receptor, ErbB1 (2, 3). The mitogenic potential of Epigen is enhanced by its unusually long persistence on the membrane before ubiquitylation and receptor-mediated depletion (3).

  1. Schneider, M.R. and E. Wolf (2010) J. Cell. Physiol. 218:460.
  2. Strachan, L. et al. (2001) J. Biol. Chem. 276:18265.
  3. Kochupurakkal, B.S. et al. (2005) J. Biol. Chem. 280:8503.
  4. Sahin, U. and C.P. Blobel 2007) FEBS Lett. 581:41.
  5. Dahlhoff, M. et al. (2010) J. Invest. Dermatol. 130:623.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Epigen Antibody (MAB11271)(1)

We have publications tested in 1 confirmed species: Guinea Pig.

We have publications tested in 1 application: In Vivo.


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(1)
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Bioinformatics

Gene Symbol Epgn
Uniprot