Recombinant Human Ephrin-B1 Protein

Product Discontinued

Recombinant Human Ephrin-B1 Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 28-237 of Human Ephrin-B
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MLAKNLEPVS WSSLNPKFLS GKGLVIYPKI GDKLDIICPR AEAGRPYEYY KLYLVRPEQA AACSTVLDPN VLVTCNRPEQ EIRFTIKFQE FSPNYMGLEF KKHHDYYITS TSNGSLEGLE NREGGVCRTR TMKIIMKVGQ DPNAVTPEQL TTSRPSKEAD NTVKMATQAP GSRGSLGDSD GKHETVNQEE KSGPGASGGS SGDPDGFFNS K

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: EFNB1
• Purity: >95% pure by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays. Use in In vitro assay reported in scientific literature (PMID 24825906)
• Theoretical MW: 25.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Human Ephrin-B1 Protein

• Cek5-L
• EFL3
• EFL-3
• EFNB1
• ELK ligand
• ELK-L
• EphrinB1
• Ephrin-B1
• LERK-2
• STRA-1

Background

Ephrin-B1, also known as EFNB1, belongs to the Eph family. Ephrins, which act as ligands for Eph receptors, are cell-surface proteins that fall into two categories, ephrin-A and ephrin-B, based on their structure and function. EFNB1 proteins are transmembrane and have conserved cytoplasmic tyrosine residues that are phosphorylated upon interaction with an EphB receptor. Also, EFNB1 transduces outside-in signals through C-terminal protein interactions that effect integrin-mediated cell attachment and migration. Recombinant human EFNB1 fused to His-tag at N-terminus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Ephrin-B1 Recombinant Protein (NBP1-72437)(1)

Publication using NBP1-72437

• Cho HJ, Hwang YS, Mood K et al. EphrinB1 Interacts with CNk1 and Promotes Cell Migration through JNk Activation. J. Biol. Chem. 2014-05-13 [PMID: 24825906] (In vitro)

Bioinformatics

• Gene Symbol: EFNB1