Ephrin-A4 Antibody (135305) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 135305
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Ephrin-A4 Antibody (135305) [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Ephrin-A4
  Leu26-Gly171
  Accession # P52798
• Specificity: Detects human Ephrin-A4 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant mouse (rm) Ephrin-A4 is observed and no cross-reactivity with recombinant human Ephrin-A3, -A5, -B3, rmEphrin-
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ephrin-A4 Antibody (135305) [Alexa Fluor® 488]

• EFL4
• EFL-4
• EFNA4
• EPH-related receptor tyrosine kinase ligand 4
• EphrinA4
• Ephrin-A4
• EPLG4MGC125826
• LERK-4
• LERK4FLJ57652
• ligand of eph-related kinase 4

Background

Ephrin-A4, also known as LERK-4 and EFL-4, (1) is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A4 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1 (2, 3). The extracellular domains of human and mouse Ephrin-A4 share 80% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.