EphB6 Antibody (92224) [Alexa Fluor® 350]

• Reactivity: Mu
• Applications: WB
• Clone: 92224
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 350

EphB6 Antibody (92224) [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse EphB6
  Leu33-Ser587
  Accession # O08644
• Specificity: Detects mouse EphB6 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human EphA1, recombinant mouse (rm) EphA2, rmEphA3, rmEphA4, recombinant rat (rr) EphA5, rmEphA6, rmEphA7, rmEphA8, rrEphB1, rmE
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphB6 Antibody (92224) [Alexa Fluor® 350]

• EC 2.7.10.1
• EPH receptor B6
• EphB6
• ephrin type-B receptor 6
• Hep
• HEPTyrosine-protein kinase-defective receptor EPH-6
• Mep
• MGC129910
• MGC129911

Background

EphB6, also known as Mep (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. However, it has been found that EphB6 contains substitutions within the kinase domain which results in EphB6 having no kinase activity (4). The ligands which bind EphB6 are unknown (2, 3). However, we have observed that the ephrin-B1 and ephrin-B2 ligands can bind the immobilized receptor in an ELISA-type assay. The extracellular domains of human and mouse EphB6 share 92% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.