EphA7 Antibody

Product Discontinued

EphA7 Antibody Summary

• Immunogen: Purified recombinant mouse EphA7 extracellular domain expressed in mouse NSO cells
• Localization: Cell Membrane
• Specificity: Anti-Mouse EphA7 recognizes recombinant mouse EphB7 by immunoblotting and ELISA. The antibody shows ~2% cross-reactivity with recombinant mouse EphA3 and less than 1% cross-reactivity with recombinant mouse EphA2, EphA4, EphA6, and EphA8 in ELISA assays.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EPHA7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.5-1 ug/ml
  • Western Blot 0.1-0.2 ug/ml
• Application Notes: ELISA: Use at a concentration of 0.5 - 1 ug/ml. The detection limit for recombinant mouse EphA7 is approximately 0.6 ng/well.WB: Use at a concentration of 0.1 - 0.2 ug/ml. The detection limit for recombinant mouse EphA7 is approximately 0.5 ng/lane under non-reducing and reducing conditions.Predicted molecular weight: 110 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Mouse.Expected to cross-react with Human (97% identity with immunogen) due to sequence homology.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EphA7 Antibody

• Cek11
• Ebk
• EC 2.7.10
• EC 2.7.10.1
• Ehk3
• EHK-3
• EK11
• EPH homology kinase 3
• Eph homology kinase-3
• EPH receptor A7
• EphA7
• EPH-like kinase 11
• ephrin type-A receptor 7
• Hek11
• Mdk1
• receptor protein-tyrosine kinase HEK11
• tyrosine-protein kinase receptor EHK-3

Background

EphA7, also known as Mdk1, Hek11, Ehk3, Ebk, and Cek11, is a member of the Eph receptor family, which binds members of the ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region is a juxtamembrane region with two tyrosines, the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the C-terminus, the latter including one conserved tyrosine. The extracellular domains of human and mouse EphA7 share 97% amino acid identity. The calculated molecular mass of the reduced mouse EphA7/Fc monomer is 85.7 kDa, but as a result of glycosylation, recombinant EphA7/Fc migrates as an approximately 110 kDa protein under reducing conditions in SDS-PAGE.Ligand recognition and binding leads to activation of the intrinsic kinase activity. EphA7 binds to ephrin A1, ephrin A2, ephrin A3, ephrin A4, and ephrin A5. Only membrane-bound or Fc-clustered ligands have been shown to activate the receptor in vitro. Soluble monomeric ligands can bind the receptor, but do not induce receptor autophosphorylation and activation. The ephrin ligands and eph receptors display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands. Ephs and ephrins play a significant role in angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPHA7
• Entrez:
  • 2045(Human)
  • 13841(Mouse)