EphA3 Antibody (99437) [Alexa Fluor® 647]

• Reactivity: Mu
• Applications: WB
• Clone: 99437
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 647

EphA3 Antibody (99437) [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse EphA3
  Glu21-Val541 (predicted)
  Accession # P29319
• Specificity: Detects mouse EphA3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) EphA1, recombinant mouse (rm) EphA2, A4, A6, A7,A8, recombinant rat (rr) EphA5, rrEphB1, rmEphB2, B3, or B6 is observe
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphA3 Antibody (99437) [Alexa Fluor® 647]

• Cek4
• EC 2.7.10
• EC 2.7.10.1
• EK4
• EPH receptor A3
• EphA3
• EPH-like kinase 4
• ephrin type-A receptor 3
• ETK
• Hek4
• HEKETK1eph-like tyrosine kinase 1
• human embryo kinase 1
• TYRO4 protein tyrosine kinase
• Tyro4
• Tyrosine-protein kinase receptor ETK1
• Tyrosine-protein kinase TYRO4

Background

EphA3, also known as Cek4, Mek4, Hek, Tyro4, and Hek4 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA3 has been shown to bind ephrin-A5, ephrin-A2, ephrin-A3, ephrin-A1, ephrin-A4, and ephrin-B1 (2, 3). The extracellular domains of mouse and human EphA3 share greater than 96% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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